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Test Code HIV12D or LAB3243 HIV 1/2 Antibody Differentiation, Serum

Important Note

HIV12D is only available as a reflex test from Preliminary Positive HIV Screen (LAB473) samples. 

Specimen Requirements

Container Type:

Optimal: Gold 

Acceptable: Red.

 
Optimal Collection Volume: 

4.5mL; full tube
Minimum Volume:

1 mL whole blood
Collection Instructions:

Gently invert the sample 5-6 times after collection.
Specimen Transport:

Refrigerate
Processing Instructions: 

1. Allow the specimen to clot for 30 minutes.

2. Centrifuge specimen within 2 hours of collection.

3. After centrifugation, specimen tubes without a gel barrier should have the serum aliquoted to a false bottom container.

4. Keep the serum refrigerated until testing can be performed.

Specimen Stability

Specimen Type Temperature Time
Serum Refrigerated 4 weeks
Room Temperature 7 days
Frozen 3 months

Specimen Rejection Criteria

Hemolysis Mild OK; Moderate OK; Gross Reject
Lipemia Mild OK; Moderate OK; Gross Reject
Icterus Mild OK; Moderate OK; Gross Reject
Other Quantity not sufficient

Useful For

Confirmation and differentiation of individual antibodies to Human Immunodeficiency Virus Types 1 and 2 (HIV-1 and HIV-2).

Methodology

Bio-Rad Geenius - Immunochromatographic assay

Reference Ranges

Negative

Interpretive

HIV-1 Result HIV-2 Result Result Reported
Negative Negative Negative
Indeterminate Negative HIV-1 Indeterminate
Negative Indeterminate* HIV-2 Indeterminate
Indeterminate Indeterminate Indeterminate
Positive Negative HIV-1 Positive
Positive Indeterminate HIV-1 Positive, HIV-2 Indeterminate
Negative Positive HIV-2 Positive
Indeterminate Positive HIV-1 Indeterminate, HIV-2 Positive
Positive Positive HIV-2 Positive with HIV-1 Cross-Reactivity
Positive Positive HIV Positive, Untypable

 

* = HIV-2 Indeterminate test results for samples from persons without any risk factors for HIV-2 infections should be confirmed by retesting with a new test cassette before reporting.

Day(s) Performed

Monday through Friday, day shift

Expected TAT

Same day or 1-2 days

Performing Laboratory

Bronson Laboratory, Chemistry - Kalamazoo

Sample Retention Time

7 days

CPT Code

  • HIV-1 antibody: 86701
  • HIV-2 antibody: 86702

LOINC Code

69668-2