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HelpTest Code HIV or LAB473 HIV Screen, Serum
Specimen Requirements
| Container Type: |
Optimal: Gold Acceptable: Red.
|
| Optimal Collection Volume: |
4.5mL; full tube |
| Minimum Volume: |
1 mL whole blood |
| Collection Instructions: |
Gently invert the sample 5-6 times after collection. |
| Specimen Transport: |
Refrigerate |
| Processing Instructions: |
1. Allow the specimen to clot for 30 minutes. 2. Centrifuge specimen within 2 hours of collection. 3. After centrifugation, specimen tubes without a gel barrier should have the serum aliquoted to a false bottom container. 4. Keep the serum refrigerated until testing can be performed. |
Specimen Stability
|
Specimen Type |
Temperature |
Time |
|---|---|---|
|
Serum |
Refrigerated |
4 weeks |
|
Room Temperature |
7 days |
|
|
Frozen |
3 months |
Specimen Rejection Criteria
|
Hemolysis |
Mild OK; Moderate OK; Gross Reject |
|
Lipemia |
Mild OK; Moderate OK; Gross Reject |
|
Icterus |
Mild OK; Moderate OK; Gross Reject |
|
Other |
Quantity not sufficient |
Useful For
Aiding in the diagnosis of HIV-1 and/or HIV-2 infection, including acute or primary HIV-1 infection. This assay may also be used as an aid in the diagnosis of HIV-1/HIV-2 infection in subjects greater than 2 years of age and pregnant women.
Methodology
Roche Cobas - Electrochemiluminescence immunoassay, 4th generation HIV 1/2 antibody and p24 antigen
Reference Ranges
Negative
Note: Preliminary positive specimens reflex the BioRad antibody differentiation HIV/1/2 test.
Interpretive
The following comments are appended to negative or preliminary positive results.
Negative
A screening test was performed with the Roche Elecsys HIV Combi 4th generation immunoassay for the qualitative determination of p24 antigen and antibodies to the Human Immunodeficiency virus types 1, 2, and group O. Currently, available assays for the detection of p24 antigen and/or antibodies to HIV may not detect all infected individuals. A negative test result does not exclude the possibility of exposure to or infection with HIV.
Preliminary Positive
Result Details: The result of this screening test is positive. When an HIV screening test is positive, it is often because the patient is infected with HIV, but additional testing is needed for confirmation. False positive results can occur due to technical issues or when the patient has unrelated medical conditions which interfere with the test. A false positive screening test is when a person not infected with HIV receives a positive screening test result.
Testing Details: A screening test was performed with the Roche Elecsys HIV Combi 4th Generation immunoassay for the qualitative determination of p24 antigen and antibodies to the Human Immunodeficiency Virus types 1, 2, and group O. This test does not differentiate between p24 antigen and HIV antibody. Per CDC recommendations, additional confirmatory testing will be performed, beginning with an HIV 1/2/ antibody differentiation immunoassay. If that test is negative, further testing for HIV RNA by PCR on a newly collected blood sample may be indicated.
Day(s) Performed
24/7
Available STAT 24/7 only for post blood/body fluid exposure and OB delivery workups.
Expected TAT
Same day. Routine results are typically reported within 2 hours of receipt in the laboratory.
Performing Laboratory
Bronson Laboratory, Chemistry - Kalamazoo and Battle Creek
Sample Retention Time
7 days
CPT Code
87389
LOINC Code
56888-1