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HelpTest Code TCP T-Cell Subsets, Naive, Memory, and Activated, Blood
Reporting Name
T Cell Phenotyping, AdvancedUseful For
Determining the presence of naive, memory, and activated T cells in various clinical contexts including autoimmune diseases, immunodeficiency states, T-cell recovery post-hematopoietic stem cell transplant, DiGeorge syndrome, and as a measure for T-cell immune competence
Naive T-cells results can be used as a surrogate marker for thymic-derived T-cell reconstitution, when used in conjunction with assessment of T-cell receptor excision circles (TREC / T-Cell Receptor Excision Circles [TREC] Analysis, Blood)
Assessing a patient's relative risk for infections
Evaluation of patients with cellular or combined primary immunodeficiencies
Evaluation of T-cell reconstitution after hematopoietic stem cell transplant, chemotherapy, biological therapy, immunosuppression, or immunomodulator therapy
Evaluation of patients with autoimmune diseases
Evaluation of HIV-positive patients for naive and memory subsets
Evaluation of T-cell immune competence (presence of memory and activated T cells) in patients with recurrent infections
Method Name
Flow Cytometry
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Whole Blood EDTAAdvisory Information
This assay provides quantitative information on various T-cell subsets in blood; it does not provide any information on the antigen-specific or otherwise functional state of the T cells. To assess the overall functional state of T cells, LPMGF / Lymphocyte Proliferation to Mitogens, Blood and LPAGF / Lymphocyte Proliferation to Antigens, Blood (using Candida and tetanus antigens) are appropriate. To assess cytomegalovirus (CMV)-specific immune competence, order CMVC8 / Cytomegalovirus (CMV) CD8 T-Cell Immune Competence, Quantitative Assessment by Flow Cytometry, Blood.
Shipping Instructions
Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. Collect and package specimen as close to shipping time as possible.
It is recommended that specimens arrive within 24 hours of collection.
Samples arriving on the weekend and observed holidays may be canceled.
Necessary Information
Ordering physician's name and phone number are required.
Specimen Required
For serial monitoring, it is recommended that specimens are collected at the same time of day.
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions: Send specimen in original tube. Do not aliquot.
Specimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Ambient | 72 hours | PURPLE OR PINK TOP/EDTA |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
Reference Values
The appropriate age-related reference values will be provided on the report.
Day(s) and Time(s) Performed
Monday through Friday
Do not send specimen after Thursday. Specimen must be received by 10 a.m. on Friday.
Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
86356 x 7
86360
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TCP | T Cell Phenotyping, Advanced | 96493-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 609282 | CD4 (T Cells) | 24467-3 |
| 609283 | CD8 (T Cells) | 14135-8 |
| 29151 | %CD4+CD45RA+ naive T cells | 89360-2 |
| 29152 | %CD4+CD62L+CD27+ naive T cells | 89340-4 |
| 29153 | %CD8+CD45RA+ naive T cells | 82744-4 |
| 29154 | %CD8+CD62L+CD27+naive T cells | 89339-6 |
| 29155 | %CD4+CD45RO+ memory T cells | 89362-8 |
| 29156 | %CD4+CD62L+CD27+CD45RO+ (Tcm) | 89338-8 |
| 29157 | %CD4+CD62L-CD27-CD45RO+ (Tem) | 89337-0 |
| 29158 | %CD8+CD45RO+ memory T cells | 89336-2 |
| 29159 | %CD8+CD62L+CD27+CD45RO+ (Tcm) | 89335-4 |
| 29160 | %CD8+CD62L-CD27-CD45RO+ (Tem) | 89334-7 |
| 29161 | %Activated CD4 T cells (4+CD25+) | 89431-1 |
| 29162 | %CD4+HLA DR+CD28+ T cells | 89333-9 |
| 29163 | %CD8+HLA DR+CD28+ T cells | 89332-1 |
| 29164 | CD4+CD45RA+ naive T cells | 26759-1 |
| 29165 | CD4+CD62L+CD27+ naive T cells | 89331-3 |
| 29166 | CD8+CD45RA+ naive T cells | 82743-6 |
| 29167 | CD8+CD62L+CD27+naive T cells | 89330-5 |
| 29168 | CD4+CD45RO+ memory T cells | 85792-0 |
| 29169 | CD4+CD62L+CD27+CD45RO+ (Tcm) | 89329-7 |
| 29170 | CD4+CD62L-CD27-CD45RO+ (Tem) | 89328-9 |
| 29171 | CD8+CD45RO+ memory T cells | 85790-4 |
| 29172 | CD8+CD62L+CD27+CD45RO+ (Tcm) | 96492-4 |
| 29173 | CD8+CD62L-CD27- CD45RO+ (Tem) | 89327-1 |
| 29174 | Activated CD4 T cells (4+CD25+) | 26982-9 |
| 29175 | CD4+HLA DR+CD28+ T cells | 89326-3 |
| 29176 | CD8+HLA DR+CD28+ T cells | 89325-5 |
| 29178 | Interpretation | 69052-9 |