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Test Code HCVB or LAB868 Hepatitis C Antibody, Serum

Important Note

Note: For hemolyzed or cadaver specimens, order the Mayo test HCCAD   Mayo Cadaver Hep C test

Patient Preparation: For 12 hours before HCVB do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins. Biotin can interfere with many immunoassay tests.

HCVB is also available as a part of the Hepatitis Panel, Acute (LAB551)

Note: Equivocal or Positive samples should be confirmed by Hepatitis C Virus RNA Quantitation by PCR. A reflex order for HCVQ (Hep C RNA Quant) is automatically placed. The HCVQ assay is performed at BMH in the microbiology department.

Specimen Requirements

Container Type:

Optimal: Gold 

Acceptable: Red.

 
Optimal Collection Volume: 

4.5mL; full tube
Minimum Volume:

1 mL whole blood
Collection Instructions:

Gently invert the sample 5-6 times after collection.
Specimen Transport:

Refrigerate
Processing Instructions: 

1. Allow the specimen to clot for 30 minutes.

2. Centrifuge specimen within 2 hours of collection.

3. After centrifugation, specimen tubes without a gel barrier should have the serum aliquoted to a false bottom container.

4. Keep the serum refrigerated until testing can be performed.

Specimen Stability

Specimen Type Temperature Time
Serum Refrigerated 21 days
Room Temperature 3 days
Frozen 3 months

Specimen Rejection Criteria

Hemolysis Mild OK; Moderate OK; Gross Reject
Lipemia Mild OK; Moderate OK; Gross Reject
Icterus Mild OK; Moderate OK; Gross Reject
Other Quantity not sufficient

Useful For

Assay results, along with other laboratory results and clinical information, may aid in the presumptive diagnosis of HCV infection in persons with signs and symptoms of hepatitis and persons at risk for hepatitis C infection. The test does not determine the state of infection or associated disease.

Methodology

Roche Cobas - Electrochemiluminescence immunoassay

Reference Ranges

Negative

 

Note: Equivocal or Positive samples should be confirmed by Hepatitis C Virus RNA Quantitation by PCR. A reflex order for HCVQ (Hep C RNA Quant) is automatically placed. The HCVQ assay is performed at BMH in the microbiology department.

Day(s) Performed

24/7

Expected TAT

Same day

Performing Laboratory

Bronson Laboratory, Chemistry - Kalamazoo and Battle Creek

Sample Retention Time

7 days

CPT Code

86803

LOINC Code

16128-1

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