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Test Code HCVQ Hepatitis C Virus RNA Detection and Quantification by PCR, Serum

Important Note

Specimen Volume: This test requires a FULL SST tube to obtain 1.5 mL serum required for testing.

Additional Codes

EPIC Code
LAB3526
Sunquest Code
HCVQ

Ordering Guidance

For detection and quantification of HCV RNA in serum before, during, and after antiviral therapy for chronic hepatitis C.

Transport Instructions

1. Transport specimen refrigerated.

2. If transport will be delayed for more 24 hours, freeze serum at -20 to -80° C (up to 60 days).

Specimen Required

Supplies: Aliquot Tube, 5 mL

Collection Container/Tube: Serum gel

Specimen Volume: 1.5 mL

Collection Instructions:

1. Centrifuge blood collection tube per collection tube manufacturer's instructions.

2. Pour off serum into aliquot tube.

Additional Information:

1.  Molecular Viral Load testing must have its own tube.

2. To avoid co-mingling and cross contamination:  If specimen is to be shared with antoher department, the molecular testing must take an aliquot of the specimen first or perform their testing prior to routing to additional testing departments.

Bronson Laboratory Services Note:

This test requires a FULL SST tube to obtain 1.5 mL serum required for testing.

Useful For

Detection of acute hepatitis C virus (HCV) infection before the appearance of HCV antibodies in serum (ie, <2 months from exposure)

 

Detection and confirmation of chronic HCV infection

 

Quantification of HCV RNA in serum of patients with chronic HCV infection (HCV antibody-positive)

 

Monitoring disease progression in chronic HCV infection and response to antiviral therapy

 

Determining cure and detection of relapse after completion of antiviral therapy

Method Name

Real-Time Transcription-mediated amplification-TMA (RT-PCR)

Hologic Genprobe Aptima HCV Quant Dx Assay

Specimen Type

Serum SST (Full)  1.5 mL Serum

Specimen Minimum Volume

0.8 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum SST Frozen (preferred) 60 days
  Refrigerated 

5 days

Reference Values

Undetected

Day(s) Performed

Monday, Thursday

CPT Code Information

87522