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Test Code AMKOL or LAB145 Amikacin Level, Serum or Plasma

Specimen Requirements

Container Type:

Optimal: SST or Mint*

*For STAT requests

Acceptable: Red or Green
Optimal Collection Volume: 

4.5mL; full tube
Minimum Volume:
  • Adults: 1 mL whole blood
  • Neonates: 1 microtainer (400-600 uL)
Collection Instructions:

Gently invert the sample 5-6 times after collection.
Specimen Transport:

Refrigerate
Processing Instructions: 

1. If serum, allow the specimen to clot for 30 minutes.

2. Centrifuge specimen within 2 hours of collection.

3. After centrifugation, specimen tubes without a gel barrier should have the serum or plasma aliquoted to a false bottom container.

4. Keep the serum or plasma refrigerated until testing can be performed.

Specimen Stability

Specimen Type Temperature Time
Serum* Refrigerated 2 days
Room Temperature 8 hours
Frozen 1 month

*Note: Heparinized plasma in gel separator tubes is less stable than serum and is only suitable for add-on testing up to 2 days following sample collection.

Specimen Rejection Criteria

Hemolysis Mild OK; Moderate OK; Gross Reject
Lipemia Mild OK; Moderate OK; Gross Reject
Icterus Mild OK; Moderate OK; Gross Reject
Other Quantity not sufficient

Useful For

Amikacin is a semi-synthetic aminoglycoside that exhibits bactericidal activity against a wide range of pathogens, including many organisms resistant to other aminoglycosides.

 

Determination of serum or plasma drug levels is required to achieve optimum therapeutic efficacy and minimize toxicity.

Methodology

Roche Cobas - Immunoassay

Reference Ranges

 Drug level   Reference Range and Reporting Units
Peak 20-25 mcg/mL
Trough 5-10 mcg/mL

Interpretive

Peak: PAMIK - Clinical & Interpretive: Amikacin, Peak, Serum (mayocliniclabs.com)

Trough: TAMIK - Clinical & Interpretive: Amikacin, Trough, Serum (mayocliniclabs.com)

Day(s) Performed

24/7

Expected TAT

Same day

Performing Laboratory

Bronson Laboratory, Chemistry - Kalamazoo

Sample Retention Time

7 days

CPT Code

80150

LOINC Code

35669-1