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Test Code VB6 Pyridoxal 5-Phosphate, Plasma

Important Note

Centrifuge at 4° C within 2 hours of collection, then aliquot all plasma into amber vial (or light protected tube).

Collection Instructions:

1. Draw blood in a chilled syringe; place specimen in a chilled, 3-mL green-top (Li Heparin) tube and mix well. Alternatively, draw blood directly in a chilled, Green top Lithium Heparin tube.

2. Immediately place Green top tube into an ice-water bath until thoroughly cooled.

3. Immediately centrifuge using a refrigerated centrifuge and aliquot plasma into an amber plastic vial (or light protected tube).

Note: If a refrigerated centrifuge is unavailable, chill the centrifuge carriers before use. Centrifuge specimen for 5 minutes or less, then promptly aliquot plasma into an amber plastic vial (or light protected tube).

4. Immediately freeze plasma.

Additional Codes

Mayo Code
PLP
EPIC Code
LAB120
Sunquest Code
VB6

 

Useful For

Determining vitamin B6 status, including in persons who present with progressive nerve compression disorders, such as carpal tunnel and tarsal tunnel syndromes

 

Determining the overall success of a vitamin B6 supplementation program

 

Diagnosis and evaluation of hypophosphatasia

Reporting Name

Pyridoxal 5-Phosphate (PLP), P

Specimen Type

Plasma Heparin
Bronson Laboratory Services Note:

Plasma Separator Tube (PST) gel tube is acceptable for this test per Kim Terrio, Mayo technical specialist.


Shipping Instructions


Ship specimen in amber vial to protect from light.



Specimen Required


Patient Preparation:

1. Fasting: 12 hours, required; Infants should have specimen collected before next feeding, water may be taken as needed

2. For 24 hours before specimen collection, patient should not take multivitamins or vitamin supplements.

Supplies: Amber Frosted Tube, 5 mL (T915)

Collection Container/Tube:

Preferred: Green top (sodium or lithium heparin) or Plasma gel separator (PST)

Acceptable: None

Submission Container/Tube: Amber vial

Specimen Volume: 1 mL

Collection Instructions:

1. Within 2 hours of collection, centrifuge at 4° C.

2. Aliquot all plasma into amber vial and freeze immediately.


Specimen Minimum Volume

0.75 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Heparin Frozen 29 days LIGHT PROTECTED

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

5-50 mcg/L

Day(s) Performed

Monday through Thursday, Saturday, Sunday

Report Available

2 to 7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

84207

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PLP Pyridoxal 5-Phosphate (PLP), P 30552-4

 

Result ID Test Result Name Result LOINC Value
4047 Pyridoxal 5-Phosphate (PLP), P 30552-4

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.