Specimen Required

Lavender Top, K2-EDTA Tube

1. Collect blood in K2-EDTA tube
   • Do not pool blood draws
   • Gently invert the tube 8-10 times after draw
2. Centrifuge within 24 hours of draw
3. Transfer plasma
   • Transfer a minimum of 0.7 mL of plasma into a sterile tube
   • Do not disturb the buffy coat when transferring
     • Label the tube with the patient’s first and last name, a unique patient identifier (DOB or MRN), and the date and time of specimen collection

Plasma Preparation Tube (PPT) Pearl top (ALTERNATE TUBE)

• Collect blood in PPT
  • 5 mL of whole blood in BD Vacutainer^® PPT
  • Gently invert the tube 8-10 times after draw
  • Label the tube with the patient’s first and last name, a unique patient identifier (DOB or MRN), and the date and time of specimen collection
• Centrifuge within 6 hours of draw
  • Do not transfer plasma
  • If the specimen is unlikely to reach Karius within 96 hours of collection, freeze specimen directly in PPT after centrifugation
  • If the gel plug does not rise to separate the cells from the plasma, the tube should be re-centrifuged at a higher speed

Specimen Stability
(After plasma has been separated from cells)

• Store at -20˚C: 6 months (If sending a frozen sample, DRY ICE MUST BE USED TO SHIP)