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Test Code PFA Platelet Function Analysis, Blood

Important Note

Specimen must be tested within 4 hours of draw.

DO NOT SPIN

Keep Ambient

Due to transport and time limitations and sample requirements, this test should only be collected at
BMH main campus. 

However, if a patient presents at an offsite draw location to be drawn for this test, DO NOT TURN THE PATIENT AWAY.  Call the courier office for a STAT pickup.

 BBC LAB:  Sample should be sent to BMH with the next courier run. If next courier run will not be within an hour, call the courier office at 341-7551 for a STAT pickup. Call BMH Hematology at 341-8620 as soon as possible to notify them the test is coming.

BLH LAB:  Test must be scheduled for collection M-F 1130 to 1215.  Follow collection instructions listed above.  Call BMH Hematology as soon as possible to notify them test is coming 341-8620.  Send with 1300 BMH courier pickup.

BSH LAB:  Should a patient present to be drawn for this test, call the courier office for a STAT pickup 341-7551 and call BMH Hematology as soon as possible to notify them a test is coming 341-8620.

Methodology

Siemens PFA Analyzer: Adhesion and aggregation response of platelets to Epinephrine and ADP 

 

Testing begins wtih the Collagen/Epinephrine (EPI) test, which can detect platelet dysfunction induced by intrinsic platelet defects, von Willebrand disease or exposure to platelet inhibiting medications.  If the EPI test is elevated,  the Collagen/ADP test is reflex ordered.   The ADP test is used to indicate if an abnormal result obtained with the EPI test may have been caused by the effect of acetyl salicylic acid (ASA) or medications containing (ASA).

Performing Laboratory

Bronson Methodist Hospital Laboratory-Coagulation Department

Specimen Requirements

Specimen must be tested within 4 hours of draw.

 

Specimen Type: Whole blood

 

Container/Tube:     2-mL Greiner Vacuette light blue citrate tubes

                                1.8 ml or 2.7 ml BD Vacutainer tubes are also acceptable

 

Specimen Volume:  2 full tubes

 

Collection Instructions:

  • Tubes must be full to fill line.
  • Gently invert 5 to 10 times to mix blood.
  • Do not centrifuge. 
  • Do not refrigerate or freeze specimen.
  • If collected through a blood collection set (butterfly), a discard tube must first be drawn to eliminate dead space from tubing.
  • Causes for rejection include tube not filled to line, clotted specimen, refrigerated or frozen specimen.

See "Important Note" at top of this page for additional information on laboratory site specific collection options. 

 

Specimen Transport Temperature

Ambient YES

Refrigerate NO

Frozen NO

Reference Values

Collagen/Epinephrine Reference Range:   70 - 170 seconds.   

If the result is <171 seconds, no further testing is indicted and the following comment is reported

Platelet function appears normal. If patient history or physical examination gives strong indication of a bleeding disorder, suggest repeat testing for confirmation. If repeat results remain normal, consider other causes of bleeding.

If the result is >170 seconds, the Collagen/ADP test is run.

 

Collagen/ADP Reference Range:  54-121 seconds.

If the result is <122 seconds the following comment is reported: 

Platelet function appears abnormal. This pattern is most commonly seen in patients with won Willebrand’s disease or congenital platelet defects. When evaluated in conjunction with clinical history and other test findings, this may indicate the need for further diagnostic testing.

If the result is <122, the following comment is reported:

Results suggest drug induced platelet dysfunction. This is most commonly seen after aspirin ingestion.

 

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

85576 X 1 if only EPI test is run

85576 x 2 if reflex to ADP test is also run