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HelpTest Code NGAML Next-Generation Sequencing, Acute Myeloid Leukemia, 11-Gene Panel, Varies
Additional Codes
RUNX1
Useful For
Evaluation of acute myeloid leukemia (AML) using a focused 11-gene panel at the time of diagnosis or possibly at relapsed/refractory disease, to assist in appropriate classification, prognosis, and therapeutic management of patients
Evaluation to determine if a different gene mutation profile is present at the time of AML relapse
Testing Algorithm
See Targeted Genes Interrogated by Next-Generation Sequencing, Acute Myeloid Leukemia, 11-Gene Panel in Special Instructions for a list of the genes and exons targeted by this assay.
Special Instructions
Method Name
Somatic Mutation Detection by Next-Generation Sequencing (NGS), Hematologic Neoplasms
Reporting Name
Next Gen Sequencing, AML, 11 GeneSpecimen Type
VariesAdvisory Information
This test is a subset of the NGSHM / OncoHeme Next-Generation Sequencing for Myeloid Neoplasms test and focuses more specifically on the gene mutations that are most prevalent and clinically significant in acute myeloid leukemias (AML). If a wider gene mutation analysis is desired, or the indication is for a myeloid malignancy other than AML, then NGSHM / OncoHeme Next-Generation Sequencing (NGS), Hematologic Neoplasms should be considered.
Shipping Instructions
Bone marrow and peripheral blood specimens must arrive within 14 days of collection.
Necessary Information
The following information is required:
1. Clinical diagnosis
2. Pertinent clinical history, including disease phase (diagnostic, remission, relapse/refractory) and therapy status (especially if patient has received a hematopoietic stem cell transplant).
3. Clinical or morphologic suspicion
4. Date of collection
5. Specimen source
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Bone marrow aspirate (preferred)
Container/Tube:
Preferred: Lavender top (EDTA) or yellow top (ACD)
Acceptable: Green top (heparin), but not preferred
Specimen Volume: 2 mL
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Send specimen in original tube.
3. Label specimen as bone marrow.
Specimen Stability: Ambient (preferred)/Refrigerate
Specimen Type: Peripheral blood
Container/Tube:
Preferred: Lavender top (EDTA) or yellow top (ACD)
Acceptable: Green top (heparin), but not preferred
Specimen Volume: 3 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send specimen in original tube.
3. Label specimen as blood.
Specimen Stability: Ambient (preferred)/Refrigerate
Specimen Type: Extracted DNA from blood or bone marrow
Container/Tube: 1.5-2 mL tube with indication of volume and concentration of the DNA
Specimen Volume: Entire specimen
Collection Instructions: Label specimen as extracted DNA and source of specimen
Specimen Stability: Frozen (preferred) /Refrigerated/Ambient
Specimen Minimum Volume
Blood, Bone Marrow: 1 mL
Extracted DNA: 100 mcL at 20 ng/mcL
concentration
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Varies | 14 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Other | Bone marrow biopsies Slides Paraffin shavings or frozen tissues and paraffin-embedded tissues Paraffin-embedded bone marrow aspirates Moderately to severely clotted |
Reference Values
An interpretive report will be provided.
Day(s) and Time(s) Performed
Monday, Wednesday, Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
81450
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| NGAML | Next Gen Sequencing, AML, 11 Gene | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| MP038 | Specimen Type | 31208-2 |
| 43554 | NGAML Result | No LOINC Needed |
| 43488 | Pathogenic Mutations Detected | 82939-0 |
| 43487 | Interpretation | 69047-9 |
| 43489 | Clinical Trials | 82786-5 |
| 43490 | Variants of Unknown Significance | 93367-1 |
| 43491 | Additional Notes | 48767-8 |
| 43492 | Method Summary | 49549-9 |
| 43493 | Disclaimer | 62364-5 |
| 43494 | AML Panel Gene List | 36908-2 |
| 43495 | Reviewed By | 19139-5 |
Forms
1. Hematopathology Patient Information (T676) in Special Instructions.
2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.