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Test Code MSCSF Bacteria, Virus, Fungus, and Parasite Metagenomic Sequencing, Spinal Fluid


Necessary Information


The following ask-at-order entry question must be answered at the time of test ordering:

Which CSF Collection Vial Number Sent (Required, Vial 1 is not acceptable)

 

Note: Answers may include "Vial 2", " Vial 3", or " Vial 4".

Test requests for specimens from  "Vial 1" will be canceled. Test requests for submitted specimens with no response or response that does not specify the collection vial number will result in testing delays while the submitting facility is contacted to provide the information.



Specimen Required


Container/Tube: Sterile vial

Specimen Volume: 2 mL

Collection Instructions:

1. Masks should be worn by those collecting and processing specimens for this assay.

2. Handle all vials under sterile technique when open to the air.

3. A separate collection vial of CSF is preferred.

4. Submit specimen from collection vial 2, 3, or 4, as specimens from vial 1 are not acceptable.

5. Indicate on the label which vial is being submitted.

6. Do not centrifuge or heat inactivate.


Useful For

Detecting and identifying pathogenic organisms including bacteria, fungi, DNA viruses, RNA viruses, and parasites in cerebrospinal fluid

 

This test is not recommended as a test of cure because nucleic acids may persist after successful treatment.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
SPID2 Specimen Identification by PCR No, (Bill Only) No

Testing Algorithm

For more information see the Meningitis/Encephalitis Panel Algorithm.

Reporting Name

Metagenomic Sequencing, CSF

Specimen Type

CSF

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
CSF Frozen (preferred) 21 days
  Refrigerated  7 days

Reject Due To

Gross hemolysis Reject
Shunt fluid Reject
Heat-inactivated specimen Reject

Reference Values

Negative.

No pathogenic DNA virus detected.

No pathogenic RNA virus detected.

No pathogenic parasite detected.

No pathogenic bacterium detected.

No pathogenic fungus detected.

Day(s) Performed

Monday through Friday

Report Available

7 to 14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MSCSF Metagenomic Sequencing, CSF 103566-6

 

Result ID Test Result Name Result LOINC Value
MSCSF Metagenomic Sequencing, CSF 103566-6

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

CPT Code Information

0480U