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Test Code LYMEAB or LAB3274 Lyme Antibody Screen, Total (IgG, IgM), Serum

Important Note

It is the first line test to order on a suspect case.

  • This test should not be used as a screening procedure for the general population.
  • This test should not be used for treatment monitoring.

If the Lyme Antibody (IgG, IgM) result is positive or equivocal, a confirmatory test is sent to Mayo Medical reference lab, TLYME, Lyme IgM and IgG, Whole Cell Sonicate, ELISA

Specimen Requirements

Container Type:

Optimal: Gold

Acceptable: Red 

 
Optimal Collection Volume: 

4.5mL; full tube
Minimum Volume:

2 mL
Collection Instructions:

Gently invert the sample 5-6 times after collection.
Specimen Transport:

Refrigerate
Processing Instructions: 

1. Allow the specimen to clot for 30 minutes.

2. Centrifuge specimen within 2 hours of collection.

3. After centrifugation, specimen tubes without a gel barrier should have the serum aliquoted to a false bottom container.

4. Keep serum refrigerated until testing can be performed.

Specimen Stability

Specimen Type Temperature Time
Serum Refrigerated 7 days
Room Temperature 5 days

Specimen Rejection Criteria

Hemolysis Mild OK; Moderate OK; Gross Reject
Lipemia Mild OK; Moderate OK; Gross Reject
Icterus Mild OK; Moderate OK; Gross Reject
Other Quantity not sufficient

Useful For

This assay is useful for the diagnosis of Lyme disease.

  • This assay is the first line test to order on a suspected case of Lyme disease.  

Methodology

Bio-Rad BioPlex 2200: Multiplex flow immunoassay

Reference Ranges

Test ID Reference Range and Reporting Units
Negative Equivocal Positive
LYMEAB < 0.8 AI 0.9-1.0 AI > 1.1 AI

AI = Antibody Index

Day(s) Performed

Monday through Friday

Expected TAT

  • LYMEAB: Same day or 1-2 days
  • TYLME (Mayo): 1-5 days

Performing Laboratory

Bronson Laboratory, Chemistry - Kalamazoo

Sample Retention Time

7 days

CPT Code

86618

LOINC Code

20449-5