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Test Code Human Papillomavirus (HPV) DNA -Lab4 (HPV) Human Papillomavirus (HPV) DNA, High Risk

Important Note

Note: The Pathology request form has been revised to reflexively order the high-risk HPV DNA test on all Pap specimens with a diagnosis of atypical squamous cells of undetermined significance (ASCUS). Medical necessity for reflexive HPV testing in women with ASCUS is supported by the ALTS trial (ASCUS/low grade squamous intraepithelial lesion triage study) and is accepted by the American Society of Colposcopy and Cervical Pathology. ThinPrep and HPV DNA testing can be ordered separately on the request form by the ordering clinician.

 

ASCCP Algorithm for Managing Abnormal Cervical Cancer Screening Tests

 

Methodology

Qualitative in-vitro test for the detection of Human
Papillomavirus in patient specimens. The test utilizes
amplification of target DNA by the Polymerase Chain Reaction (PCR)
and nucleic acid hybridization for the detection of 14 high-risk
(HR) HPV types in a single analysis. The test specifically
identifies types HPV 16 and HPV 18 while concurrently detecting the
other high risk types (31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66
and 68).

Performing Laboratory

Bronson Laboratory Services-Cytology

Specimen Requirements

Specimen source is required.

 

Submit the following specimen:

 

Specimen Type: Ectocervix and
endocervix

Container/Tube: ThinPrep Pap
PreservCyt solution vial

Specimen Volume: Entire collection
(min 1 mL)

Collection Instructions:

1. Obtain adequate specimen from ectocervix using a
plastic spatula.

2. Rinse spatula as quickly as possible into PreservCyt
solution vial by swirling spatula vigorously in vial 10
times.

3. Discard spatula.

4. Obtain an adequate sampling from endocervix
using an endocervical brush device.

5. Insert brush into cervix until only bottom-most
fibers are exposed. Slowly rotate 1/4 or 1/2 turn in 1 direction.
Do not over-rotate.

6. Rinse brush as quickly as possible in PreservCyt
solution by rotating device in solution 10 times while pushing
against PreservCyt vial wall. Swirl brush vigorously to
further release material.

7. Discard brush.

8. Label specimen with patient’s full name, date
of birth, date of collection, and source of specimen.

 

 

 

Specimen Transport Temperature

Ambient 2 weeks/Refrigerate 3 weeks

Reference Values

An interpretive report will be provided.

 

ASCCP
Algorithm for Managing Abnormal Cervical Cancer Screening
Tests

Day(s) Test Set Up

Monday through Friday

Test Classification and CPT Coding

87624