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Test Code HSTNT2 or LAB3596 Troponin T, high sensitivity, 2 hours after initial draw, plasma

Important Note

This order code should only be used for the second draw following an initial troponin high-sensitivity order (LAB2674).

The primary use of the troponin assay is to detect the presence of an active acute coronary syndrome (ACS). Since a positive test result could constitute a medical emergency, patients should be tested for this condition in a monitored setting.

If you suspect an outpatient may have an ACS and feel ordering a troponin is warranted, it is strongly recommended that the patient be directed to a higher level of care for ongoing monitoring.

For additional information, Under "Resources" on this page, see the Labwire article describing changes in test methodology (from Generation 4) effective 11/5/18 and an update effective 7/16/19

Specimen Requirements

***This test is to be collected 2 hours after the initial troponin draw. If the sample is collected less than 90 minutes after the draw, the test will be canceled (unless specifically requested by a clinician). This is because the reference range for the "delta" (the time between the initial and 2 hours)  is based upon studies at 2 hours. Samples collected too soon may give a false impression of the significance of the change in values.***

 

Container Type:

 Optimal: Mint

 

Acceptable: Green

Optimal Collection Volume: 

4.5mL; full tube

Minimum Volume:

1 mL whole blood

Collection Instructions:

Gently invert the sample 5-6 times after collection.

Specimen Transport:

Refrigerate

Processing Instructions: 
  1. Centrifuge specimen immediately upon receipt. 
  2. After centrifugation, specimen tubes without a gel barrier should have the plasma aliquoted to a false bottom container.

Specimen Stability

Specimen Type Temperature Time
Plasma Refrigerated 24 hours
Frozen 12 months

Specimen Rejection Criteria

Hemolysis Mild OK; Moderate OK; Gross Reject
Lipemia Mild OK; Moderate OK; Gross Reject
Icterus Mild OK; Moderate OK; Gross Reject
Other Quantity not sufficient

Useful For

This assay is intended to be used as an aid in the diagnosis of myocardial infarction.

Methodology

Roche Cobas - Electrochemiluminescence immunoassay

Reference Ranges

Gender

Reference Range and Reporting Units

Male

< 22 ng/L

Female

< 14 ng/L

  • Delta change from the initial troponin draw at 2 hours : < 8 ng/L

Day(s) Performed

24/7

Expected TAT

Same day

Performing Laboratory

Bronson Laboratory, Chemistry - Kalamazoo, Battle Creek, Paw Paw, South Haven

Sample Retention Time

7 days

CPT Code

84484