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Test Code HBSB or LAB472 Hepatitis B Surface Antibody, Serum

Specimen Requirements

Container Type:

Optimal: Gold 

Acceptable: Red.

 
Optimal Collection Volume: 

4.5mL; full tube
Minimum Volume:

1 mL whole blood
Collection Instructions:

Gently invert the sample 5-6 times after collection.
Specimen Transport:

Refrigerate
Processing Instructions: 

1. Allow the specimen to clot for 30 minutes.

2. Centrifuge specimen within 2 hours of collection.

3. After centrifugation, specimen tubes without a gel barrier should have the serum aliquoted to a false bottom container.

4. Keep the serum refrigerated until testing can be performed.

Specimen Stability

Specimen Type Temperature Time
Serum Room Temp 4 days
Refrigerated 6 days
Frozen 3 months

Specimen Rejection Criteria

Hemolysis Mild OK; Moderate OK; Gross Reject
Lipemia Mild OK; Moderate OK; Gross Reject
Icterus Mild OK; Moderate OK; Gross Reject
Other Quantity not sufficient

Useful For

Assay results may be used as an aid in the determination of susceptibility to hepatitis B virus (HBV) infection for individuals prior to or following HBV vaccination or where vaccination status is unknown.

Methodology

Roche Cobas - Electrochemiluminescence immunoassay

Reference Ranges

The accepted criterion for immunity to HBV is ≥ 10 mIU/mL of anti-HBs as defined by the WHO Reference Preparation. 

HBSB Result Comment Added
< 10 mIU/mL NOTE: Anti-HBs is below 10 mIU/mL. Patient is not considered to have protective immunity to Hepatitis B Infection.
≥ 10 mIU/mL NOTE: Anti-HBs is greater than or equal to 10 mIU/mL. Patient is considered to have protective immunity to Hepatitis B Infection.

 

Interpretive

A result > 10 mIU/mL indicates antibodies to hepatitis B resulting from vaccination or past infection, which indicates immunity to reinfection.

Day(s) Performed

24/7

Expected TAT

Same day

Performing Laboratory

Bronson Laboratory, Chemistry - Kalamazoo

Sample Retention Time

7 days

CPT Code

86706

LOINC Code

16935-9