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HelpTest Code EBVB or LAB863 Epstein-Barr Virus Antibody Panel, Serum
Profile Information
| Test ID | Reporting Name | Available Separately? | Always Performed? |
|---|---|---|---|
|
EBVCAG |
EBV VCA IgG |
N |
Y |
|
EBVNAG |
EBV NA IgG |
N |
Y |
|
EBVEAG |
EBV EA (Early Antigen) IgG |
N |
Y |
|
EBVCAM |
EBV VCA IgM |
N |
Y |
|
EBVHM |
EBV Heterophile Antibody |
N |
Y |
|
EBVB Interpretive Report |
N |
Y |
|
Specimen Requirements
| Container Type: |
Optimal: Gold Acceptable: Red
|
| Optimal Collection Volume: |
4.5mL; full tube |
| Minimum Volume: |
2 mL |
| Collection Instructions: |
Gently invert the sample 5-6 times after collection. |
| Specimen Transport: |
Refrigerate |
| Processing Instructions: |
1. Allow the specimen to clot for 30 minutes. 2. Centrifuge specimen within 2 hours of collection. 3. After centrifugation, specimen tubes without a gel barrier should have the serum aliquoted to a false bottom container. 4. Keep serum refrigerated until testing can be performed. |
Specimen Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated | 7 days |
| Room Temperature | 8 hours |
Specimen Rejection Criteria
| Hemolysis | Mild OK; Moderate OK; Gross Reject |
| Lipemia | Mild OK; Moderate OK; Gross Reject |
| Icterus | Mild OK; Moderate OK; Gross Reject |
| Other | Quantity not sufficient |
Useful For
Detection and monitoring of acute or chronic Epstein-Barr virus (EBV) infection progression. This panel includes EBV IgG antibodies to Nuclear Antigen-1, Viral Capsid Antigen, and Early Antigen, and EBV IgM antibodies to Viral Capsid Antigen and Heterophile.
Methodology
Bio-Rad BioPlex 2200: Multiplex flow immunoassay
Reference Ranges
| Test ID | Reference Range and Reporting Units | ||
|---|---|---|---|
| Negative | Equivocal | Positive | |
| EBV VCA IgG | < 0.8 AI | 0.9-1.0 AI | > 1.1 AI |
| EBV NA IgG | < 0.8 AI | 0.9-1.0 AI | > 1.1 AI |
| EBV EA (Early Antigen) IgG | < 0.8 AI | 0.9-1.0 AI | > 1.1 AI |
| EBV VCA IgM | < 0.8 AI | 0.9-1.0 AI | > 1.1 AI |
| EBV Heterophile Antibody | < 0.8 AI | 0.9-1.0 AI | > 1.1 AI |
AI = Antibody Index
Note: An interpretation of the antibody pattern is included on each report.
Interpretive
EBV status can be applied to a patient based on the results of standard tests.
- In acute IM, both EBV IgM and EBV IgG antibodies to viral capsid antigen (VCA) rise rapidly.
- EBV VCA IgM antibody disappears over about four weeks.
- Heterophile antibodies of the IgM class are normally present in acute IM only. A rise is indicative of an acute stage of infection. The presence of antibodies in otherwise healthy individuals usually indicates immunological exposure as silent primary infection. Antibody levels tend to rise and peak after 3 – 4 weeks, then decline and usually dissipate after 2 - 3 months.
- EBV EA-D IgG antibody shows a transient rise during acute infection and becomes undetectable after 3-6 months. IgG antibodies to EA-D are frequently present in acute IM and generally absent in convalescence. A rise may indicate acute infection, reactivation, or chronic infection. The presence of antibodies in otherwise healthy individuals usually indicates reactivation, especially when paired with rising NA-1 levels.
- EBV NA-1 IgG antibody usually appears 3 months after initial infection and typically remains for life, as well as EBV VCA IgG
Day(s) Performed
Monday through Friday
Expected TAT
Same day or 1-2 days
Performing Laboratory
Bronson Laboratory, Chemistry - Kalamazoo
Sample Retention Time
7 days
CPT Code
- EBVCAG - 86665
- EBVNAG - 86664
- EBVEAG - 86663
- EBVCAM - 86665