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HelpTest Code DSDNA or LAB2161 dsDNA Antibody, Serum
Specimen Requirements
| Container Type: |
Optimal: Gold Acceptable: Red
|
| Optimal Collection Volume: |
4.5mL; full tube |
| Minimum Volume: |
1 mL |
| Collection Instructions: |
Gently invert the sample 5-6 times after collection. |
| Specimen Transport: |
Refrigerate |
| Processing Instructions: |
1. Allow the specimen to clot for 30 minutes. 2. Centrifuge specimen within 2 hours of collection. 3. After centrifugation, specimen tubes without a gel barrier should have the serum aliquoted to a false bottom container. 4. Keep serum refrigerated until testing can be performed. |
Specimen Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated | 7 days |
| Room Temperature | 8 hours |
Specimen Rejection Criteria
| Hemolysis | Mild OK; Moderate OK; Gross Reject |
| Lipemia | Mild OK; Moderate OK; Gross Reject |
| Icterus | Mild OK; Moderate OK; Gross Reject |
| Other | Quantity not sufficient |
Useful For
This assay is helpful as an aid in the diagnosis of systemic autoimmune diseases.
Methodology
Bio-Rad BioPlex 2200: Multiplex flow immunoassay
Reference Ranges
| Test ID | Reference Range and Reporting Units |
|---|---|
| DSDNA | Negative: < 5 IU/mL |
| Intermediate: 5-9 IU/mL | |
| Positive: > 9 IU/mL |
Interpretive
A positive result for double-stranded DNA (dsDNA) IgG antibodies in the appropriate clinical context is suggestive of systemic lupus erythematosus (SLE). The performance characteristics of dsDNA IgG antibodies in SLE is dependent on the immunological method used for their detection, the patient’s disease state including clinical manifestations, and demographics.
Weak-positive dsDNA IgG antibody results have a low-positive predictive value for SLE.
Negative results do not rule out a diagnosis of SLE.
Source: Mayo Medical Labs
Day(s) Performed
Monday through Friday
Expected TAT
Same day or 1-2 days
Performing Laboratory
Bronson Laboratory, Chemistry - Kalamazoo
Sample Retention Time
7 days
CPT Code
86225
LOINC Code
5130-0