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HelpTest Code CAFF or LAB706 Caffeine Level, Serum
Specimen Requirements
| Container Type: |
Optimal: Gold Acceptable: Red |
| Optimal Collection Volume: |
4.5mL; full tube |
| Minimum Volume: |
1 mL whole blood or 1 microtainer (400-600 uL) |
| Collection Instructions: |
Gently invert the sample 5-6 times after collection. |
| Specimen Transport: |
Refrigerate |
| Processing Instructions: |
1. Allow the specimen to clot for 30 minutes. 2. Centrifuge specimen within 2 hours of collection. 3. After centrifugation, specimen tubes without a gel barrier should have the serum aliquoted to a false bottom container. 4. Keep the serum refrigerated until testing can be performed. |
Specimen Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated | 3 days |
Specimen Rejection Criteria
| Hemolysis | Mild OK; Moderate OK; Gross Reject |
| Lipemia | Mild OK; Moderate OK; Gross Reject |
| Icterus | Mild OK; Moderate OK; Gross Reject |
| Other | Quantity not sufficient |
Useful For
This assay is useful in monitoring the therapy of caffeine administration in cases of neonatal apnea.
Methodology
Roche Cobas - Homogeneous enzyme immunoassay
Reference Ranges
Therapeutic range: 5-20 ug/mL
Critical Value
| Age | Critical Value |
|---|---|
| 0-3 months | > 30 ug/mL |
Day(s) Performed
24/7
Expected TAT
Same day
Performing Laboratory
Bronson Laboratory, Chemistry - Kalamazoo
Sample Retention Time
7 days
CPT Code
80155
LOINC Code
3422-3