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Test Code APCRR or LAB3496 Activated Protein C Resistance Ratio

Specimen Requirements

Specimen Type:

Whole Blood

Container Type:

2.7 mL or 1.8 mL blue top tube:

Optimal Collection Volume:

  • 2.7 mL in solid blue top tube or 1.8 mL in clear blue top tube.  The correct fill volume is indicated by the etched line on the tube.
  • Click here to view the specimen collection guide for blue top (citrate) tubes.

Collection Instructions:

  1. Gently invert 5 to 10 times to mix blood.
  2. If collected through a blood collection set (butterfly), a discard tube must first be drawn to eliminate dead space from tubing.  

Specimen Transport Temperature:

  • Room temperature up to 24 hours. If testing is delayed, separate plasma and freeze (see outpatient collection instructions below.
  • Specimens cannot be refrigerated.

Processing Instructions:

  1. Centrifuge sample within 4 hours of collection.  Platelet poor plasma must be obtained per laboratory procedure.
  2. If testing will be delayed >4 hours from collection, aliquot plasma into separate vial and freeze immediately.

Outpatient collections

  1. Spin down sample, remove plasma, and spin plasma again to obtain platelet-poor plasma.
  2. Separate platelet-poor plasma into a screw-top vial.
  3. Freeze plasma immediately.

Specimen Stability

Specimen Type

Temperature

Time

Whole blood

Room Temperature

4 hours

Platelet Poor Plasma

Room Temperature

4 hours

Platelet Poor Plasma

Frozen 

18 months

Specimen Rejection Criteria

Hemolysis

Hemoglobin up to 500 mg/dL

Lipemia

Triglycerides up to 1791 mg/dL

Icterus

Bilirubin up to 18.8 mg/dL

Useful For

  • Evaluation of patients with incident or recurrent venous thromboembolism (VTE).
  • Evaluation of individuals with a family history of VTE.

Methodology

Optical clot based assay

Reference Ranges

Test ID

Reference Range

APCRR

≥ 2.26 (ratio)

Day(s) Performed

Testing performed Monday/Wednesday/Friday 0800-1200

Performing Laboratory

Bronson Laboratory, Coagulation - Kalamazoo

Sample Retention Time

Ambient samples

24 hours

Frozen samples

1 month

CPT Code

85307

LOINC Code

13590-5