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Test Code DCOMX DAT-Complement

Important Note

Only available when polyspecific DAT is positive.

Additional Codes

Epic Order Code: LAB3532
Sunquest Order Code: DCOMX

Specimen Required

Patient Preparation:  
Container Type:

Optimal:  Pink Top (EDTA)

Acceptable: Lavender

Optimal Collection Volume: 

6.0mL whole blood

Collection Instructions:

Collect specimen and gently invert several times to mix. Label the specimen, in the presence of the patient, with full name, date of birth and patient medical record number. 

Processing Instructions: 

1. Collect sample in pink top container.

2. Label per policy in the presence of the patient.

3. Send whole blood sample to Blood Bank for testing.

Useful For

A complement DAT test is only performed by BMH dayshift and is performed when ordered as separate test or as part of a transfusion reaction investigation. Testing used to determine the specificty of the C3 immunoglobulin coating the red cell.

Method Name


Stability Information

Specimen Type Temperature Time
  Refrigerated 3 days
  Ambient  1 day

Rejection Due To

Hemolysis Mild OK; Moderate OK; Gross Reject
Lipemia Mild OK; Moderate OK; Gross Reject
Icterus Mild OK; Moderate OK; Gross Reject
Other N/A


Post Processing Specimen Type

Whole Blood

Specimen Minimum Volume

Collecting minimum volumes can result in a need for sample recollection, and/or a delay in results. Minimum volumes are subjective and cannot account for all aspects of specimen and testing needs. Refer to the Specimen Required section for optimal volumes for laboratory specimens. Contact the Bronson Laboratory if complex collection exceptions occur that require more information.


Minimum Volume: 3.0mL whole blood

Performing Laboratory

Bronson Laboratory, Laboratory-Kalamazoo

Day(s) and Time(s) Performed

Monday through Sunday--day shift only

CPT Code Information

86880-Coombs, direct


Sample Retention Time

Blood Bank specimens (other than Cord Blood) are retained in the Blood Bank for 30 days. Pretransfusion testing should be completed within 2 days of collection.