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Test Code DAT Direct coombs (DAT), polyspecific with reflex to IgG

Additional Codes

Epic Order Code: LAB274
Sunquest Order Code: DAT

Specimen Required

Patient Preparation:

 
Container Type:

Optimal: Pink Top (EDTA)

               

Acceptable:  Lavender

                    
Optimal Collection Volume: 

1.5mL Cord Blood                                                                    

6mL whole blood 

0.5mL heel stick
Collection Instructions:

Collect specimen and gently invert several times to mix. Label the specimen, in the presence of the patient, with full name, date of birth and patient medical record number. 
Processing Instructions: 

1. Collect sample in pink top container.

2. Label per policy in the presence of the patient.

3. Send whole blood sample to Blood Bank for testing.

Useful For

A positive direct Coombs’ test will be evaluated to determine the specificity of the immunoglobulin coating the red cell (IgG and/or C3). A positive polyspecific DAT will automatically reflex to an IgG DAT.

A complement DAT test is only performed by BMH dayshift and is performed when ordered as separate test or as part of a transfusion reaction investigation. For testing on infants, see CDWK - Cord Blood Testing for Suspected HDN. Infant's red cells will only be tested for IgG coating.

Method Name

Gel or Tube

Stability Information

Specimen Type Temperature Time
        Whole blood/Cord Blood Refrigerated 1 day
  Ambient  1 day

Rejection Due To

Hemolysis Mild OK; Moderate OK; Gross Reject
Lipemia Mild OK; Moderate OK; Gross Reject
Icterus Mild OK; Moderate OK; Gross Reject
Other Improperly labeled specimens

 

Post Processing Specimen Type

Whole Blood

 

 

Specimen Minimum Volume

Collecting minimum volumes can result in a need for sample recollection, and/or a delay in results. Minimum volumes are subjective and cannot account for all aspects of specimen and testing needs. Refer to the Specimen Required section for optimal volumes for laboratory specimens. Contact the Bronson Laboratory if complex collection exceptions occur that require more information.

 

Minimum Volume: 3 mL whole blood
Neonate Volume:

1.5 mL cord blood

0.5 mL heel stick

Performing Laboratory

Bronson Laboratory, Blood Bank-Kalamazoo and Battle Creek

Day(s) Performed

24/7

LOINC Code Information

 

Test ID Test Order Name Order LOINC Value
    1007-4

CPT Code Information

86880-Coombs, direct

86850-Antibody screen (if appropriate)

Specimen Retention Time

7 days

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