Test Code UFHXA or LAB3473 Unfractionated Heparin Anti-Xa Assay
Specimen Requirements
Specimen Type: |
Whole Blood |
Container Type: |
Blue top: 2.7 mL or 1.8 mL |
Optimal Collection Volume: |
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Collection Instructions: |
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Specimen Transport Temperature: |
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Processing Instructions: |
Outpatient collections
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Specimen Stability
Specimen Type |
Temperature |
Time |
---|---|---|
Whole blood |
Room Temperature |
1 hour |
Platelet Poor Plasma |
Room Temperature |
4 hours |
Platelet Poor Plasma |
Frozen at - 20 C |
2 weeks |
Specimen Rejection Criteria
- Tube underfilled
- Refrigerated specimen
- Clotted specimen
- Specimen not processed within four hours of collection
Useful For
Measuring heparin concentration:
- In the presence of prolonged baseline activated partial thromboplastin time (APTT) (eg, lupus anticoagulant, "contact factor" deficiency, etc)
- When unfractionated heparin dose needed to achieve desired APTT prolongation is unexpectedly higher (>50%) than expected
Not useful for monitoring therapy with the heparinoid "danaparoid"
Methodology
The Liquid Anti-Xa kit (ACL TOP) is a 1-stage chromogenic assay based on a synthetic chromogenic substrate and on factor Xa inactivation.(Package insert HemosIL Liquid Anti-Xa)
Reference Ranges
Therapeutic Range
- 0.30-0.70 IU/mL for treatment of DVT/PE
- 0.30-0.50 IU/mL for cardiac/neuro protocols
Day(s) Performed
24/7
Expected TAT
Same day
Performing Laboratory
Bronson Laboratory, Coagulation - Kalamazoo and Battle Creek
Sample Retention Time
24 hours
CPT Code
85520