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Test Code UFHXA or LAB3473 Unfractionated Heparin Anti-Xa Assay

Important Note

This assay should only be ordered when monitoring levels of unfractionated heparin.

If monitoring levels of Low Molecular Weight Heparin, please order the Low Molecular Weight Heparin Anti-Xa assay.

Specimen Requirements

Specimen Type:

Whole Blood

Container Type:

Blue top: 2.7 mL or 1.8 mL

Optimal Collection Volume:

  • 2.7 mL in solid blue top tube or 1.8 mL in clear blue top tube.  The correct fill volume is indicated by the etched line on the tube.
  • Click here to view the specimen collection guide for blue top (citrate) tubes.

Collection Instructions:

  1. Do not draw from an arm with a heparin lock.
  2. Gently invert 5 to 10 times to mix blood.
  3. If collected through a blood collection set (butterfly), a discard tube must first be drawn to eliminate dead space from tubing.  

Specimen Transport Temperature:

  • Room temperature for up to 4 hours. If testing is delayed, separate plasma and freeze (see outpatient collection instructions below).
  • Specimens cannot be refrigerated

Processing Instructions:

  1. Centrifuge sample within 4 hours of collection. Platelet-poor plasma must be obtained per laboratory procedure.
  2. If testing will be delayed >4 hours from collection, aliquot plasma into a separate vial and freeze immediately.

Outpatient collections

  1. Spin down the sample, remove plasma, and spin plasma again to obtain platelet-poor plasma.
  2. Separate the platelet-poor plasma into a screw-top vial.
  3. Freeze plasma immediately.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Specimen Stability

Specimen Type

Temperature

Time

Whole blood

Room Temperature

1 hour

Platelet Poor Plasma

Room Temperature

4 hours

Platelet Poor Plasma

Frozen at - 20 C

2 weeks

Specimen Rejection Criteria

  • Tube underfilled
  • Refrigerated specimen
  • Clotted specimen
  • Specimen not processed within four hours of collection

Useful For

Measuring heparin concentration:

  • In the presence of prolonged baseline activated partial thromboplastin time (APTT) (eg, lupus anticoagulant, "contact factor" deficiency, etc)
  • When unfractionated heparin dose needed to achieve desired APTT prolongation is unexpectedly higher (>50%) than expected

Not useful for monitoring therapy with the heparinoid "danaparoid"

Methodology

The Liquid Anti-Xa kit (ACL TOP) is a 1-stage chromogenic assay based on a synthetic chromogenic substrate and on factor Xa inactivation.(Package insert HemosIL Liquid Anti-Xa)

Reference Ranges

Therapeutic Range

  • 0.30-0.70 IU/mL for treatment of DVT/PE
  • 0.30-0.50 IU/mL for cardiac/neuro protocols

Day(s) Performed

24/7

Expected TAT

Same day

Performing Laboratory

Bronson Laboratory, Coagulation - Kalamazoo and Battle Creek

Sample Retention Time

24 hours

CPT Code

85520