Test Code TBBS Quantitative Lymphocyte Subsets: T, B, and Natural Killer (NK), Blood
Additional Codes
Mayo Test ID |
---|
TBBS
Epic code LAB2265 |
Sunquest Code |
TBBS |
Reporting Name
QN Lymphocyte Subsets: T, B, and NKUseful For
Serial monitoring of CD4 T-cell count in HIV-positive patients
Follow-up and diagnostic evaluation of primary immunodeficiencies, including severe combined immunodeficiency
Immune monitoring following immunosuppressive therapy for transplantation, autoimmunity, and other immunological conditions where such treatment is utilized
Assessment of immune reconstitution post hematopoietic cell transplantation
Early screening of gross quantitative anomalies in lymphocyte subsets in infection or malignancies
Absolute quantitation of circulating B cells for diagnosis of chronic lymphocytic leukemia patients as indicated in the 2008 International Workshop on Chronic Lymphocytic Leukemia guidelines
Testing Algorithm
See Newborn Screen Follow-up for Severe Combined Immunodeficiency Syndrome (SCID) in Special Instructions.
Method Name
Flow Cytometry
Performing Laboratory

Specimen Type
Whole Blood EDTAAdvisory Information
This assay should not be used for diagnosing lymphocytic malignancies or evaluation of lymphocytosis of unknown etiology, though the latter may be identified through this assay in a screening assessment. In such cases, LCMS / Leukemia/Lymphoma Immunophenotyping, Flow Cytometry, Varies will be recommended, which includes a hematopathology review. However, this assay can be used for absolute quantitation of B cells in CLL patients as indicated above.
Shipping Instructions
It is recommended that specimens arrive within 24 hours of draw. Draw and package specimen as close to shipping time as possible.
Necessary Information
Date of draw is required.
Specimen Required
For serial monitoring, we recommend that specimen draws be performed at the same time of day.
Container/Tube: 4 mL Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions: Send specimen in original tube. Do not aliquot.
Specimen Minimum Volume
1 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Whole Blood EDTA | Ambient | 52 hours | PURPLE OR PINK TOP/EDTA |
Reject Due To
Gross hemolysis | Reject |
Gross lipemia | Reject |
Other | Specimen in aliquot tube |
Special Instructions
Reference Values
The appropriate age-related reference values will be provided on the report.
Day(s) and Time(s) Performed
Monday through Sunday
Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
86355-B cells, total count
86357-Natural killer (NK) cells, total count
86359-T cells, total count
86360-Absolute CD4/CD8 count with ratio
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
TBBS | QN Lymphocyte Subsets: T, B, and NK | 80721-4 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
3321 | CD45 Total Lymph Count | 27071-0 |
3316 | % CD3 (T Cells) | 8124-0 |
3322 | CD3 (T Cells) | 8122-4 |
3319 | % CD4 (T Cells) | 8123-2 |
3325 | CD4 (T Cells) | 24467-3 |
3320 | % CD8 (T Cells) | 8101-8 |
3326 | CD8 (T Cells) | 14135-8 |
3318 | % CD19 (B Cells) | 8117-4 |
3324 | CD19 (B Cells) | 8116-6 |
4054 | % CD16+CD56 (NK cells) | 8112-5 |
4055 | CD16+CD56 (NK cells) | 20402-4 |
3327 | 4/8 Ratio | 54218-3 |
6657 | Comment | 80722-2 |