Sign in →

Test Code TBBS Quantitative Lymphocyte Subsets: T, B, and Natural Killer (NK), Blood

Important Note

**Note** Patient must arrive at draw site early! Due to Specimen Stability, must be sent to Mayo Lab same day as draw.

Do not draw on Saturdays, Sundays, Day before a Holiday or on a Holiday. Draw only Monday through Friday

Specimen should arrive at Bronson Laboratory Battle Creek Hospital Lab by 12:30 or Bronson Laboratory downtown Kalamazoo by 14:00 to allow same day sample shipment to reference lab.

Specimen should be sent to reference lab same day of collection due to limited 52 hour sample stability.

 

Additional Codes

Mayo Test ID

TBBS

 

Epic code

LAB2265
Sunquest Code
TBBS

 

Reporting Name

QN Lymphocyte Subsets: T, B, and NK

Useful For

Serial monitoring of CD4 T-cell count in HIV-positive patients

 

Follow-up and diagnostic evaluation of primary immunodeficiencies, including severe combined immunodeficiency

 

Immune monitoring following immunosuppressive therapy for transplantation, autoimmunity, and other immunological conditions where such treatment is utilized

 

Assessment of immune reconstitution post hematopoietic cell transplantation

 

Early screening of gross quantitative anomalies in lymphocyte subsets in infection or malignancies

 

Absolute quantitation of circulating B cells for diagnosis of chronic lymphocytic leukemia patients as indicated in the 2008 International Workshop on Chronic Lymphocytic Leukemia guidelines

Method Name

Flow Cytometry

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Whole Blood EDTA


Advisory Information


This assay should not be used for diagnosing lymphocytic malignancies or evaluation of lymphocytosis of unknown etiology, though the latter may be identified through this assay in a screening assessment. In such cases, LCMS / Leukemia/Lymphoma Immunophenotyping, Flow Cytometry, Varies will be recommended, which includes a hematopathology review. However, this assay can be used for absolute quantitation of B cells in CLL patients as indicated above.



Shipping Instructions


It is recommended that specimens arrive within 24 hours of draw. Draw and package specimen as close to shipping time as possible.



Necessary Information


Date of draw is required.



Specimen Required


For serial monitoring, we recommend that specimen draws be performed at the same time of day.

 

Container/Tube: 4 mL Lavender top (EDTA)

Specimen Volume: 3 mL

Collection Instructions: Send specimen in original tube. Do not aliquot.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Ambient 52 hours PURPLE OR PINK TOP/EDTA

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Other Specimen in aliquot tube

Reference Values

The appropriate age-related reference values will be provided on the report.

Day(s) and Time(s) Performed

Monday through Sunday

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

86355-B cells, total count

86357-Natural killer (NK) cells, total count

86359-T cells, total count

86360-Absolute CD4/CD8 count with ratio

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TBBS QN Lymphocyte Subsets: T, B, and NK 80721-4

 

Result ID Test Result Name Result LOINC Value
3321 CD45 Total Lymph Count 27071-0
3316 % CD3 (T Cells) 8124-0
3322 CD3 (T Cells) 8122-4
3319 % CD4 (T Cells) 8123-2
3325 CD4 (T Cells) 24467-3
3320 % CD8 (T Cells) 8101-8
3326 CD8 (T Cells) 14135-8
3318 % CD19 (B Cells) 8117-4
3324 CD19 (B Cells) 8116-6
4054 % CD16+CD56 (NK cells) 8112-5
4055 CD16+CD56 (NK cells) 20402-4
3327 4/8 Ratio 54218-3
6657 Comment 80722-2