Test Code SOMIS (Clost. botul.) Clostridium botulinum Toxin Examination
Methodology
Useful to confirm the diagnosis of botulism. Culture of
food, stool, or serum. Mouse inoculation for toxins A, B,
C,
and E. Laboratory confirmation of the presence of toxin is
performed by neutralization studies in mice. Final results will
not
be available for 7 to 10 days.
Performing Laboratory
Michigan Department of Community Health
Specimen Requirements
Testing must be preapproved. Due to costs
of
reagents and involvement of animal inoculations, all testing
for
food borne illness MUST be arranged with
MDCH Surveillance and
Infectious Disease Epidemiology Section at 517-335-8165 or
Microbiology Section Manager at 517-335-9641 prior to
collection.
Specimen source is required.
Specimen Type: Food, implicated
Container/Tube: Sterile, wide-mouth,
screw-capped jar
Specimen Volume: A minimum of 0.5 cup
Collection Instructions:
1. Label jar with patient’s full name, date of birth,
and
date and time of collection.
2. Causes for rejection include unlabeled or incomplete
request
form or specimen label not matching request form, leaking
specimen,
no preapproval for testing, or specimen submitted in an
inappropriate manner (ie, shipped incorrectly according to
Federal
standards).
Specimen Type: Serum
Container/Tube: Red top
Specimen Volume: A minimum
of 7-10
mL
Collection Instructions:
1. Label tube with patient’s full name, date of birth,
and
date and time of collection.
2. Causes for rejection include unlabeled or incomplete
request
form or specimen label not matching request form, leaking
specimen,
no preapproval for testing, or specimen submitted in an
inappropriate manner (ie, shipped incorrectly according to
Federal
standards).
Specimen Type: Unpreserved stool
Container/Tube: Plastic container
Specimen Volume: A minimum of 15-25 g
Collection Instructions:
1. Label container with patient’s full name, date of
birth, and date and time of collection.
2. Causes for rejection include unlabeled or incomplete
request
form or specimen label not matching request form, leaking
specimen,
no preapproval for testing, or specimen submitted in an
inappropriate manner (ie, shipped incorrectly according to
Federal
standards).
Specimen Transport Temperature
Refrigerate
Reference Values
See report.
Day(s) Test Set Up
Monday through Friday