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HelpTest Code MONOT with Reflex Epstein Barr (EBV) IgM Tests MONO Test with reflex Epstein-Barr Virus IgM Antibodies
Additional Codes
LAB4891
Specimen Required
| Container Type: |
Sample may be collected in any of the following tube types: Optimal:
Acceptable: |
| Optimal Collection Volume: |
3.0 mL whole blood |
| Processing Instructions: |
1. In gold SST or Red tube, allow sample to clot for 30 minutes. 2. Centrifuge specimen. 3. Separate serum into an aliquot container. |
Useful For
Testing for the presence of infectious mononucleosis heterophile antibodies as an aid in the diagnosis of infectious mononucleosis. Currently, the heterophile antibody test is the most common procedure for the diagnosis of acute infectious mononucleosis (IM). It uses mononucleosis antigens to detect IM heterophile antibodies. However, up to 20% of patients with primary EBV infection do not develop heterophile antibodies. With the introduction of EBV VCA IgM testing, the detection of acute IM has been enhanced. However, EBV VCA IgM may not be detectable in all acute IM patients, thus testing for both heterophile antibody and anti-VCA IgM increases the likelihood of detecting acute primary infection.
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| MONOT | Mono Screen | Y | Y |
| Reflex |
Epstein Barr Virus IgM Antibodies |
N | *Only when the initial screen is negative.* |
The reflex panel includes the following:
EBV VCA IgM and EBV Heterophile Ab - please see the EBVB panel for additional information on these tests.
Method Name
MONOT (Screen) - Color Immunochromatographic Dipstick Technology
EBV VCA IgM Ab and EBV Heterophile IGM - Multiplex Flow Immunoassay
Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated | 48 hours |
| Frozen | 3 months |
Rejection Due To
| Hemolysis | Mild OK; Moderate OK; Gross Reject |
| Lipemia | Mild OK; Moderate OK; Gross Reject |
| Icterus | Mild OK; Moderate OK; Gross Reject |
| Other | Incorrect tube type |
| Quantity not sufficient |
Specimen Minimum Volume
Collecting minimum volumes can result in a need for sample recollection, and/or a delay in results. Minimum volumes are subjective and cannot account for all aspects of specimen and testing needs. Refer to the Specimen Required section for optimal volumes for laboratory specimens. Contact the Bronson Laboratory if complex collection exceptions occur that require more information.
| Minimum Volume: | 1.0 mL whole blood, 0.5 mL serum |
Performing Laboratory
MONOT - Bronson Laboratory, Hematology - Kalamazoo, Battle Creek, Paw Paw, South Haven
Reflex Epstein-Barr Virus IgM Antibodies - Bronson Laboratory, Chemistry - Kalamazoo
Day(s) Performed
MONOT - 24/7
Reflex Epstein-Barr Virus IgM Antibodies - Monday through Saturday
Reference Values
MONOT
- Negative
EBV VCA IgM
- AI index < 0.8: Negative
- AI index 0.9-1.0: Equivocal
- AI index > 1.1: Positive
EBV Heterophile IgM
- AI index < 0.8: Negative
- AI index 0.9-1.0: Equivocal
- AI index > 1.1: Positive
LOINC Code Information
MONOT: 31418-7
EBV VCA IgM: 24115-8
EBV Heterophile IgM: 6425-3
CPT Code Information
86308, 86309, 86664, 86665
Sample Retention Time
7 Days