Test Code Lab13 (Fungal Stain) Cytology, Fungal Stain (Methenamine-Silver Stain)
Methodology
Grocott’s Methenamine-Silver Stain/Wright Giemsa
Stain/Papanicolaou Stain
Includes staining of a monolayer smear preparation by
ThinPrep
method with methenamine-silver, Wright Giemsa
stains, and
Papanicolaou stain.
Immunocompromised patients may develop progressive pulmonary
insufficiency from pulmonary infiltrates due to
Pneumocystis, fungus, cytomegalovirus (CMV), or herpes
virus infections. Open lung biopsy or bronchoscopy may be
required
to detect the organism so that appropriate therapy may be
instituted in these life-threatening situations.
Performing Laboratory
Bronson Laboratory Services-Cytology
Specimen Requirements
Specimen Type: Bronchoalveolar lavage
(preferred), sputum, or bronchial washing
Container/Tube: CytoLyt solution
container
Specimen Volume: Entire collection
Collection Instructions:
1. Physician aseptically collects specimen.
2. Label container with patient’s full name, date of
birth,
date of collection, and source of specimen.
3. Send specimen with completed requisition to the
laboratory
immediately at ambient temperature.
Additional Information: Causes for
rejection include improper collection or incomplete request
form.
Forms: Pathology requisition; include
patient’s full name, date of birth, specimen source, date
of
service, name special stain request, and
pertinent
clinical history
Specimen Transport Temperature
Ambient
Reference Values
Negative to positive for Pneumocystis carinii,
fungi,
CMV, herpes virus, or malignant cells
Day(s) Test Set Up
Monday through Friday