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HelpTest Code IABCS B-Cell Phenotyping Profile for Immunodeficiency and Immune Competence Assessment, Blood
Reporting Name
Immune Assessment B Cell Subsets, BUseful For
Screening for common variable immunodeficiency (CVID) and hyper-IgM syndromes
Assessing B-cell subset reconstitution after stem cell or bone marrow transplant
Assessing response to B-cell-depleting immunotherapy
Identifying defects in transmembrane activator and calcium modulator and cyclophilin ligand (CAML) interactor (TACI) and B-cell-activating factor receptor (BAFF-R) in patients presenting with clinical symptoms and other laboratory features consistent with CVID
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CVID | CVID Confirmation Flow Panel | Yes | No |
Testing Algorithm
If immune assessment B-cell subsets test is abnormal, then confirmation will be performed at an additional charge.
When multiple specimen types are required to perform a panel of tests, the laboratory will perform the tests for which the appropriate specimen type was received and the laboratory will cancel those for which the appropriate specimen was not received. Please be advised that this may change the degree of interpretation received with the report. If only the refrigerate EDTA sample is received, this test will be canceled and converted to RBCS / Relative B-Cell Subset Analysis Percentage which provides the relative B-cell subset values without quantitation.
Method Name
TBBS: Flow Cytometry
IABC: Fluorescent Flow Cytometry
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Whole Blood EDTAAdvisory Information
Shipping Instructions
Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. Draw and package specimen as close to shipping time as possible.
It is recommended that specimens arrive within 24 hours of draw.
Samples arriving on the weekend and observed holidays may be canceled.
Necessary Information
1. Date of draw is required.
2. Ordering physician's name and phone number are required.
Specimen Required
Two separate EDTA specimens are required: 1 refrigerated and 1 at ambient transport temperature.
For serial monitoring, we recommend that specimen draws be performed at the same time of day.
Specimen Type: Whole blood for TBBS / Quantitative Lymphocyte Subsets: T, B, and NK
Container/Tube: 4 mL Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions:
1. Send specimen in original tube. Do not aliquot.
2. Label specimen as blood for TBBS / Quantitative Lymphocyte Subsets: T, B, and NK.
Specimen Stability Information: Ambient <52 hours
Specimen Type: Whole blood for IABC / B-Cell Phenotyping Screen for Immunodeficiency and Immune Competence Assessment, Blood
Container/Tube: Lavender top (EDTA)
Specimen Volume:
≤14 years: 4 mL
>14 years: 10 mL
Collection Instructions:
1. Send specimen in original tube. Do not aliquot.
2. Label specimen as blood for IABC / B-Cell Phenotyping Screen for Immunodeficiency and Immune Competence Assessment, Blood.
Specimen Stability Information: Refrigerated <48 hours
Specimen Minimum Volume
TBBS: 1 mL
IABC
≤14 years: 3 mL
>14 years: 5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Varies | 48 hours | PURPLE OR PINK TOP/EDTA |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
Reference Values
The appropriate age-related reference values will be provided on the report.
Day(s) and Time(s) Performed
Monday through Friday
Specimens are required to be received in the laboratory on weekdays and by 4 p.m. on Friday. No weekend processing.
Test Classification
See Individual Test IDsCPT Code Information
T- and B-Cell Quantitation by Flow Cytometry
86355-B cells, total count
86357-Natural killer (NK) cells, total count
86359-T cells, total count
86360-Absolute CD4/CD8 count with ratio
B-Cell Phenotyping Screen for Immunodeficiency and Immune Competence Assessment, Blood
86356 x7 - Mononuclear cell antigen, quantitative
Common Variable Immunodeficiency Confirmation Flow Panel
88184-Flow cytometry, first marker (if appropriate)
88185 x 2-Flow cytometry, each additional marker (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| IABCS | Immune Assessment B Cell Subsets, B | 90416-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 30296 | CD19+ % of total Lymphocytes | 8117-4 |
| 3321 | CD45 Total Lymph Count | 27071-0 |
| 3316 | % CD3 (T Cells) | 8124-0 |
| 29094 | CD20+ % of total Lymphocytes | 8119-0 |
| 3322 | CD3 (T Cells) | 8122-4 |
| 30298 | CD27+ % of CD19+ B cells | 89358-6 |
| 3319 | % CD4 (T Cells) | 8123-2 |
| 30300 | CD27+ IgM+ IgD+ % of CD19+ B cells | 89352-9 |
| 3325 | CD4 (T Cells) | 24467-3 |
| 30302 | CD27+ IgM- IgD- % of CD19+ B cells | 89350-3 |
| 3320 | % CD8 (T Cells) | 8101-8 |
| 30304 | CD27+ IgM+ IgD- % of CD19+ B cells | 89348-7 |
| 3326 | CD8 (T Cells) | 14135-8 |
| 30306 | IgM+ % of CD19+ B cells | 89346-1 |
| 3318 | % CD19 (B Cells) | 8117-4 |
| 30308 | CD38+ IgM- % of CD19+ B cells | 89344-6 |
| 30310 | CD38+ IgM+ % of CD19+ B cells | 89341-2 |
| 3324 | CD19 (B Cells) | 8116-6 |
| 4054 | % CD16+CD56 (NK cells) | 8112-5 |
| 30312 | CD21+ % of CD19+ B cells | 89356-0 |
| 30314 | CD21- % of CD19+ B cells | 89355-2 |
| 4055 | CD16+CD56 (NK cells) | 20402-4 |
| 3327 | 4/8 Ratio | 54218-3 |
| 30297 | CD19+ | 8116-6 |
| 29095 | CD20+ | 9558-8 |
| 6657 | Comment | 80722-2 |
| 30299 | CD27+ | 89353-7 |
| 30301 | CD27+ IgM+ IgD+ | 89351-1 |
| 30303 | CD27+ IgM- IgD- | 89349-5 |
| 30305 | CD27+ IgM+ IgD- | 89347-9 |
| 30307 | IgM+ | 89345-3 |
| 30309 | CD38+ IgM- | 89343-8 |
| 30311 | CD38+ IgM+ | 89357-8 |
| 30313 | CD21+ | 25164-5 |
| 30315 | CD21- | 89354-5 |
| 30316 | Interpretation | 80722-2 |
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| TBBS | QN Lymphocyte Subsets: T, B, and NK | Yes | Yes |
| IABC | Immune Assessment B Cell Subsets, B | No | Yes |