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Test Code HIVS or LAB2544 Rapid HIV Screen (1/2 antibody and p24 antigen), Serum

Important Note

This is a visually read dipstick test where the result is read by the presence or absence of a potentially subjective line.   This test is only to be used in cases where an extremely rapid result is needed  (i.e., blood and body fluid exposure or pending OB delivery with no prenatal testing).    The standard automated HIV Test (LAB473)  is available 22 hours a day with  the exception during daily maintenance on third shift.  That test is preferable, and often meets the Turn Around Time needs for exposure and OB testing. 

Profile Information

Reporting Name Available Separately Always Performed
HIV 1/2 Antibodies N Y
HIV 1 P24 Antigen N Y

Specimen Requirements

Container Type:

Optimal: Gold 

Acceptable: Red
Optimal Collection Volume: 

5 mL whole blood
Minimum Volume:

1 mL
Collection Instructions:

Gently invert the sample 5-6 times after collection.
Specimen Transport:

Refrigerated
Processing Instructions: 
  1. Allow the sample to clot for 30 minutes.
  2. Centrifuge specimen within 2 hours of collection.
  3. After centrifugation, specimen tubes without a gel barrier should have the serum aliquoted to a false bottom container.
  4. Keep serum refrigerated until testing can be performed.

Specimen Stability

Specimen Type Temperature Time
Serum

Refrigerated

3 days

Specimen Rejection Criteria

Quantity not sufficient

Useful For

Rapid HIV screening for the detection of antibodies to HIV 1 and 2, or the p24 HIV 1 antigen.

Methodology

Abbott Determine - Immunochromatographic

Reference Ranges

Negative

Interpretive

The following comments are appended to negative or preliminary positive results.

 

HIV 1/2 Antibodies

Result

Interpretation

Negative

The screening test for HIV 1/2 antibody is negative - STAT testing was performed by a rapid HIV antibody test.

 

Please note: A negative result with any HIV antibody test does not preclude the possibility of infection with HIV.

Preliminary Positive

 Due to frequent false positives by this method, any positive results by this manual method are tested by an automated method prior to reporting. 

 

 

HIV 1 P24 Antigen

Result

Interpretation

Negative

The screening test for HIV-1 p24 antigen is negative - STAT testing was performed by a rapid HIV antigen test.

 

Please note: a negative result with any HIV antibody test does not preclude the possibility of infection with HIV.

Preliminary Positive

Due to frequent false positives by this method, any positive results by this manual method are tested by an automated method prior to reporting. 

 

Day(s) Performed

Available STAT 24/7 only for post-blood/body fluid exposure
and OB delivery workups.

Expected TAT

Same day

Performing Laboratory

Bronson Laboratory, Chemistry - Kalamazoo, Paw Paw, South Haven

Sample Retention Time

6 days

CPT Code

  • HIV 1/2 Antibody - 86703
  • HIV 1 p24 Antigen - 87899

LOINC Code

75666-8