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Test Code GBM or LAB727 Glomerular Basement Membrane (GBM) IgG, Serum

Specimen Requirements

Container Type:

Optimal: Gold

Acceptable: Red 

 

Optimal Collection Volume: 

4.5mL; full tube

Minimum Volume:

1 mL

Collection Instructions:

Gently invert the sample 5-6 times after collection.

Specimen Transport:

Refrigerate

Processing Instructions: 

1. Allow the specimen to clot for 30 minutes.

2. Centrifuge specimen within 2 hours of collection.

3. After centrifugation, specimen tubes without a gel barrier should have the serum aliquoted to a false bottom container.

4. Keep serum refrigerated until testing can be performed.

Specimen Stability

Specimen Type Temperature Time
Serum Refrigerated 7 days
Room Temperature 8 hours

Specimen Rejection Criteria

Hemolysis Mild OK; Moderate OK; Gross Reject
Lipemia Mild OK; Moderate OK; Gross Reject
Icterus Mild OK; Moderate OK; Gross Reject
Other Quantity not sufficient

Useful For

Used as an aid in the diagnosis of Goodposture's Syndrome.

Methodology

Bio-Rad BioPlex 2200: Multiplex flow immunoassay

Reference Ranges

 Test ID   Reference Range and Reporting Units
GBM Negative: < 1.0 AI
Positive: > 1.0 AI

AI = Antibody Index

Day(s) Performed

Monday through Friday

Expected TAT

Same day or 1-2 days

Performing Laboratory

Bronson Laboratory, Chemistry - Kalamazoo

Sample Retention Time

7 days

CPT Code

83516

LOINC Code

68386-2