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Test Code FT4 or LAB2388 Free T4, Serum or Plasma

Important Note

This test is recommended over total T4 because the free T4 represents the physiologically active hormone rather than the quantity of carrier proteins.

Specimen Requirements

Container Type:

Optimal: Gold or Mint*

*For STAT requests

Acceptable: Red or Green

Optimal Collection Volume: 

4.5mL; full tube

Minimum Volume:

1 mL whole blood

Collection Instructions:

Gently invert the sample 5-6 times after collection.

Specimen Transport:

Refrigerate

Processing Instructions: 

1. If serum, allow the specimen to clot for 30 minutes.

2. Centrifuge specimen within 2 hours of collection.

3. After centrifugation, specimen tubes without a gel barrier should have the serum or plasma aliquoted to a false bottom container.

4. Keep serum or plasma refrigerated until testing can be performed.

Specimen Stability

Specimen Type Temperature Time
Serum* Refrigerated 7 days
Frozen 30 days

*Note: Heparinized plasma in gel separator tubes is less stable than serum and is only suitable for add-on testing up to 2 days following sample collection.

Specimen Rejection Criteria

Hemolysis Mild OK; Moderate OK; Gross Reject
Lipemia Mild OK; Moderate OK; Gross Reject
Icterus Mild OK; Moderate OK; Gross Reject
Other Quantity not sufficient

Useful For

Free T4 is measured together with TSH when thyroid function disorders are suspected. The determination of FT4 is also suitable for monitoring thyrosuppressive therapy.

Methodology

Roche Cobas - Electrochemiluminescence assay

Reference Ranges

0.8-1.7 ng/dL

Day(s) Performed

24/7

Expected TAT

Same day

Performing Laboratory

Bronson Laboratory, Chemistry - Kalamazoo, Battle Creek, Paw Paw, South Haven

Sample Retention Time

7 days

CPT Code

84439

LOINC Code

3024-7