Ordering Guidance

It is strongly recommended that this test be used in the context of results of MYD88 / MYD88, L265P, Somatic Gene Mutation, DNA Allele-Specific PCR, Varies. If MYD88 has not been previously performed, consider LPLFX / Lymphoplasmacytic Lymphoma/Waldenstrom Macroglobulinemia, MYD88 L265P with Reflex to CXCR4, Varies during evaluation of lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia.

Shipping Instructions

Whole blood or bone marrow specimens must arrive within 10 days of collection.

Necessary Information

The following information is required:

1. Pertinent clinical history

2. Clinical or morphologic suspicion

3. Date and time of collection

4. Specimen source

Specimen Required

Submit only 1 of the following specimens:

Preferred

Specimen Type: Bone marrow aspirate

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD), green top (sodium heparin)

Specimen Volume: 2 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send bone marrow specimen in original tube. Do not aliquot.

3. Label specimen as bone marrow.

Specimen Stability Information: Ambient (preferred) 10 days /Refrigerated 10 days

Specimen Type: Whole blood

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD), green top (sodium heparin)

Specimen Volume: 3 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimen in original tube. Do not aliquot.

3. Label specimen as blood.

Specimen Stability Information: Ambient (preferred) 10 days/Refrigerated 10 days

Specimen Type: Paraffin-embedded tissue

Container/ Tube: Paraffin block

Collection Instructions:

1. Decalcified specimens (eg, bone marrow core biopsies) are not acceptable.

2. Indicate specimen source.

Specimen Stability Information: Ambient

Additional Information: If the quality of the biopsy specimen is poor, testing should not be ordered. Testing may be canceled if DNA requirements are inadequate.

Acceptable

Specimen Type: Tissue slide

Slides: 20 Unstained slides

Container/ Tube: Transport in plastic slide holders

Collection Instructions:

1. Send 20 unstained, nonbaked slides with 5-micron thick sections of tissue.

2. Decalcified specimens (eg, bone marrow core biopsies) are not acceptable.

3. Indicate specimen source.

Specimen Stability Information: Ambient

Additional Information: Testing may be canceled if resultant extracted DNA does not meet concentration requirements.

Specimen Type: Frozen tissue

Container/Tube: Plastic container

Specimen Volume: 100 mg

Collection Instructions:

1. Freeze tissue within 1 hour of collection

2. Indicate specimen source.

Specimen Stability Information: Frozen

Additional Information: Testing may be canceled if resultant extracted DNA does not meet concentration requirements.

Specimen Type: Extracted DNA

Container/Tube: 1.5- to 2-mL tube

Specimen Volume: Entire specimen

Collection Instructions:

1. DNA must be extracted within 7 days of collection.

2. Label specimen as extracted DNA and source of specimen.

3. Provide volume and concentration of DNA on label.

Specimen Stability Information: Frozen (preferred)/Refrigerated/Ambient

Additional Information: DNA must be extracted in a CLIA-certified laboratory or equivalent and must be extracted from a specimen type listed as acceptable for this test (including applicable anticoagulants). We cannot guarantee that all extraction methods are compatible with this test. If testing fails, one repeat will be attempted, and if unsuccessful, the test will be reported as failed and a charge will be applied.