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Test Code CTBID Culture Referred for Identification, Mycobacterium and Nocardia, Varies

Important Note

Effective 12/22/23

Test Code: CTB

Due to a nationwide shortage of the BD BACTEC MGIT- Growth supplement kit, broth mycobacterial cultures are temporarily unavailable. Mayo Clinic Laboratories offers our clients alternative testing methodologies.

Specimens submitted for Mycobacteria and Nocardia culture will be performed using solid culture media only. This may impact the turnaround time for positive results.

Infectious

Reporting Name

Culture Refer for ID, Mycobacterium

Useful For

Rapid identification to the species level for Mycobacterium species, Nocardia species, and other aerobic actinomycete genera and species from pure culture isolates

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RMALM Id MALDI-TOF Mass Spec AFB No, (Bill Only) No
RTBSP Id, Mtb Speciation, PCR No, (Bill Only) No
ISMY ID by 16S Sequencing No, (Bill Only) No
LCTB Id, MTB complex Rapid PCR No, (Bill Only) No

Testing Algorithm

Reflex tests may be performed at an additional charge. All mycobacteria and Nocardia (including aerobic actinomycetes) submitted will be identified and billed as appropriate.

 

See Culture Referred for Identification Mycobacterium and Nocardia Algorithm.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Shipping Instructions


1. See Infectious Specimen Shipping Guidelines.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.



Necessary Information


1. Specimen source is required.

2. Isolate description is required: Gram stain reaction, morphology, tests performed.



Specimen Required


Specimen Type: Mycobacterium species or aerobic actinomycetes organism in pure culture

Supplies: Infectious Container, Large (T146)

Container/Tube: Middlebrook (7H10 or 7H11) or Lowenstein-Jensen medium slant or in broth (eg, Mycobacteria Growth Indicator Tube [7H9] broth)

Specimen Volume:

Solid media: Visible growth of isolate

Isolate in broth media: ≥3 mL

Collection Instructions: Organism must be in pure culture, actively growing. Do not submit mixed cultures.

Additional Information: A minimum volume of 3 mL is recommended to perform all initial testing, this may include: stains, sub-culture media, nucleic acid probes, and any additional testing that may be required to determine the identification. If the broth sample volume is less than 3 mL, initial testing may be limited, and increased turnaround time is likely.


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reject Due To

Agar plate Reject
Isolates other than Mycobacterium species or aerobic actinomycetes Reject

Reference Values

Not applicable

Day(s) Performed

Monday through Sunday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

Culture Referred for Identification, Mycobacterium

87118-Identification of mycobacteria

87158-Identification of mycobacteria by other methods (if appropriate)

87118 -Id MALDI-TOF Mass Spec AFB (if appropriate)

87153-Mycobacteria Identification by Sequencing (if appropriate)

87150-Id, Mtb Speciation, PCR (if appropriate)

87150- Id, MTB complex Rapid PCR (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CTBID Culture Refer for ID, Mycobacterium 543-9

 

Result ID Test Result Name Result LOINC Value
CTBID Culture Refer for ID, Mycobacterium In Process

Report Available

60 to 70 days

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.