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HelpTest Code BCUL Blood Culture Culture, Blood
Infectious
Additional Codes
Epic Codes
For pediatric patients 6 months of age or younger: LAB46201
- 2 separate draws in a Pediatric Blood Culture bottle
For patients over 6 months of age: LAB462
- 2 separate draws in an Aerobic and Anaerobic Blood Culture bottle set
Methodology
Includes aerobic and anaerobic bacterial culture for adults,
aerobic culture for pediatrics, antimicrobial removal culture, Gram
stain of all positive cultures, and identification and
susceptibility testing of clinically significant pathogens. This
test does not include culture for mycobacteria or viruses.
NOTE: All positive blood cultures will have (Rapid Blood Culture ID) RBCID PCR performed to identify most common blood stream infection organisms and antimicrobial resistance DNA targets.
RBCID Panel detects:
Gram Positive:
Enterococcus faecalis
Enterococcus faecium
Listeria monocytogenes
Genus Staphylococcus
Staphylococcus aureus
Staphylococcus epidermidis
Staphylococcus lugdunensis
Genus Streptococcus
Streptococcus agalactiae
Streptococcus pneumoniae
Streptococcus pyogenes
Gram Negative:
Acinetobacter calcoaceticus-baumannii complex
Order Enterobacterales
Enterobacter cloacae complex
Escherichia coli
Klebsiella oxytoca
Klebsiella pneumonia group
Klebsiella aerogenes
Proteus
Salmonella species
Serratia marcescens
Bacteriodes fragilis
Haemophilus influenzae
Neisseria meningitidis(Encapsulated)
Pseudomonas aeruginosa
Stenotrophomonas maltophilia
Yeast:
Candida albicans
Candida auris
Candida glabrata
Candida krusei
Candida parapsilosis
Candida tropicalis
Cryptococcus neoformans/gattii
Antimicrobial Resistance Genes:
mecA/C-Methicillin resistance (non Staphylococcus aureus)
mecA/C and MREJ- Methicillin resistance in Staphylococcus aureus
vanA/B Vancomycin resistance
KPC - Carbapenem resistance
CTX-M- Extended-Spectrum Beta Lactamase (ESBL)
IMP - Carbapenemase resistance
NDM - Carbapenemase resistance
OXA-48-like - Carbapenemase resistance
VIM - Carbapenemase resistance
Performing Laboratory
Bronson Laboratory Services-Microbiology
Specimen Requirements
Optimal sensitivity of blood culture requires 2 sets (30-40 mL)
per day drawn from different sites.
Preliminary negative results are given after 24 and 48 hours of
incubation. Final negative reports are given after 5 days of
incubation.
Specimen source, date and time of
draw, patient’s age, physician’s name, clinical
diagnosis, and any unusual organisms suspected are
required.
Pediatric Collection
Specimen Type: Blood
Container/Tube: Pediatric (pink) blood
culture bottle
Specimen Volume: Body Weight
dependent. See below Pediatric Chart.
Collection Instructions:
1. For detailed collection instructions, see Blood Culture
Collection Procedure in Special Instructions.
2. Disinfect venipuncture site and allow to air dry. Disinfect
top of culture bottle.
3. Draw blood and inoculate directly into bottle.
Do not change needle on syringe prior to
inoculating bottle. Do not inject air into
bottle.
4. Label bottle with patient’s full name, date of
birth, and date and time of draw.
Adult Collection
Specimen Type: Blood
Container/Tube: Aerobic (silver) and
anaerobic (purple) blood culture bottles
Specimen Volume: 20 mL
Collection Instructions:
1. For detailed collection instructions, see Blood Culture
Collection Procedure in Special Instructions.
2. Disinfect venipuncture site and allow to air dry. Disinfect
tops of blood culture bottles.
3. Draw blood and inoculate 10 mL into aerobic bottle and 10 mL
into anaerobic bottle. Do not change the needle on
the syringe prior to inoculating bottles. Do not
inject air into bottles.
4. Label bottles with patient’s full name, date of
birth, and date and time of draw.
Adult Blood Volumes for Culture
| Blood Volume Drawn |
Blood Volume in Culture |
| 16-20 mL | Divide evenly into aerobic (silver) and anaerobic (purple) bottle |
| 13-16 mL | 8 mL into Aerobic (silver) bottle and 5-8 mL into Anaerobic (purple) bottle |
| 10-12 mL | 5-7 mL into Aerobic (silver) bottle and 5 mL into Anaerobic (purple) bottle |
| 3-9 mL | Entier amount into Aerobic (silver) bottle |
| NOTE: Do not collect Pediatric bottles on adult patients unless multiple attempts to collect >3mL have been made. | |
Pediatric BloodVolumes for Culture
|
Patient Weight |
Total BC volume per day in mL |
Site 1 |
Site 2 |
|||
|
Pounds |
Kilograms |
Bottle 1 |
Bottle 2 |
Bottle 1 |
Bottle 2 |
|
|
|
|
|
|
|
|
|
|
<8 |
<3.9 |
1 |
0.5 ml Peds |
|
0.5 ml Peds |
|
|
8 |
4-7.9 |
3 |
1.5 ml Peds |
|
1.5 ml Peds |
|
|
17 |
8-13.9 |
6 |
3 ml Peds |
|
3 ml Peds |
|
|
30 |
14-18.9 |
12 |
3 ml Peds |
3 ml Peds |
3 ml Peds |
3 ml Peds |
|
41 |
19-25.9 |
16 |
4 ml Peds |
4 ml Peds |
4 ml Peds |
4 ml Peds |
|
57 |
26-39.9 |
20 |
5 ml aerobic |
5 ml anaerobic |
5 ml aerobic |
5 ml anaerobic |
|
88 |
40-53.9 |
32 |
8 ml aerobic |
8 ml anaerobic |
8 ml aerobic |
8 ml anaerobic |
|
119 |
>54 |
40 |
10 ml aerobic |
10 ml anaerobic |
10 ml aerobic |
10 ml anaerobic |
Aerobic Blood Culture Bottle Anaerobic Blood Culture Bottle
Pediatric Blood Culture Bottle
Specimen Transport Temperature
Ambient 24 hours/Refrigerate NO
Reference Values
Normal finding: no growth
Abnormal finding: bacteria recovered
Critical value (automatic call-back): any positives
Day(s) Test Set Up
Monday through Sunday
Test Classification and CPT Coding
87040-Blood Culture
87150-RBCID