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Test Code ALKP or LAB112 Alkaline Phosphatase, Serum or Plasma

Specimen Requirements

Container Type:

Optimal: Gold or Mint*

*For STAT requests

Acceptable: Red or Green
Optimal Collection Volume: 

4.5mL; full tube
Minimum Volume:
  • Adults: 1 mL whole blood
  • Neonates: 1 microtainer (400-600 uL)
Collection Instructions:

Gently invert the sample 5-6 times after collection.
Specimen Transport:

Refrigerate
Processing Instructions: 

1. If serum, allow the specimen to clot for 30 minutes.

2. Centrifuge specimen within 2 hours of collection.

3. After centrifugation, specimen tubes without a gel barrier should have the serum or plasma aliquoted to a false bottom container.

4. Keep the serum or plasma refrigerated until testing can be performed.

Specimen Stability

Specimen Type Temperature Time
Serum* Refrigerated 7 days
Room Temperature 7 days
Frozen 2 months

*Note: Heparinized plasma in gel separator tubes is less stable than serum and is only suitable for add-on testing up to 2 days following sample collection.

Specimen Rejection Criteria

Hemolysis Mild OK; Moderate OK; Gross Reject
Lipemia Mild OK; Moderate OK; Gross Reject
Icterus Mild OK; Moderate OK; Gross Reject
Other N/A

Useful For

This assay is used to diagnose and monitor various liver, bone, intestinal, and parathyroid diseases.

Methodology

Roche Cobas - Spectrophotometric

Reference Ranges

Male & Female

Age Reference Range and Reporting Units
0 - 14 day 83-248 U/L
15 days to less than 1 year 122-469 U/L
1 year to less than 10 years 142-335 U/L
10 years to less than 13 years 129-417 U/L

Male

Age Reference Range and Reporting Units
13 to less than 15 years 116-468 U/L
15 to less than 17 years 82-331 U/L
17 to less than 19 years 55-149 U/L
> 19 years 40-129 U/L

Female

Age Reference Range and Reporting Units
13 to less than 15 years 57-254 U/L
15 to less than 17 years 50-117 U/L
> 17 years 35-117* U/L

Interpretive

ALP - Clinical & Interpretive: Alkaline Phosphatase, Serum (mayocliniclabs.com)

Day(s) Performed

24/7

Expected TAT

Same day

Performing Laboratory

Bronson Laboratory, Chemistry - Kalamazoo, Battle Creek, Paw Paw, South Haven

Sample Retention Time

7 days

CPT Code

84075

LOINC Code

6768-6