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HelpTest Code TCRVB TCR V-Beta Repertoire Analysis by Spectratyping, Blood
Useful For
Assessment of T-cell receptor diversity in various clinical contexts including primary immunodeficiencies, monitoring immune reconstitution posthematopoietic cell transplantation, and temporal assessment of repertoire changes in autoimmune diseases and viral infections
Testing Algorithm
Additional tests that could be ordered include:
-TREC / T-Cell Receptor Excision Circles (TREC) Analysis, Blood
-CD4RT / CD4 T-Cell Recent Thymic Emigrants (RTE)
-TCP / T-Cell Subsets, Naive, Memory, and Activated
Special Instructions
Method Name
Molecular TCR Vb-CDR3 Fragment Length Analysis
Reporting Name
TCR Spectratyping, BSpecimen Type
Whole Blood EDTAAdvisory Information
Mayo Laboratory Director/Consultant approval is required prior to ordering this test in patients >40 years of age.
Shipping Instructions
It is recommended that specimens arrive within 24 hours of draw.
Samples arriving on the weekend and observed holidays may be canceled.
Necessary Information
Ordering physician's name and phone number are required.
Specimen Required
For serial monitoring, it is recommended to perform specimen collection at the same time of day, if possible.
Supplies: Ambient Shipping Box-Critical Specimens Only (T668)
Specimen Type: Blood
Container/Tube: Lavender top (EDTA)
Specimen Volume:
Adults: 10 mL
Pediatrics:
-Preferred volume for >1 year: 3 mL
-Preferred volume for ≤1 year: 1 mL
Collection Instructions: Send specimen in original tube.
Specimen Minimum Volume
Adults: 5 mL
Pediatrics: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Ambient | 48 hours | PURPLE OR PINK TOP/EDTA |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Other | Clotted |
Reference Values
References values will be provided in the patient report.
Day(s) and Time(s) Performed
Varies
Do not draw specimen after Thursday. Specimen must be received by 10 a.m. on Friday.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
81340-TRG (T cell antigen receptor, beta) (eg, leukemia and lymphoma), gene rearrangement analysis to detect abnormal clonal population(s); using amplification methodology (eg, polymerase chain reaction)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TCRVB | TCR Spectratyping, B | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 36473 | Family 2 Diversity Ratio | 82501-8 |
| 36474 | Family 3-1 Diversity Ratio | 82500-0 |
| 36475 | Family 4 Diversity Ratio | 82499-5 |
| 36476 | Family 5 Diversity Ratio | 82498-7 |
| 36477 | Family 6 Diversity Ratio | 82497-9 |
| 36478 | Family 7 Diversity Ratio | 82496-1 |
| 36479 | Family 9 Diversity Ratio | 82495-3 |
| 36480 | Family 10 Diversity Ratio | 82494-6 |
| 36481 | Family 11 Diversity Ratio | 82493-8 |
| 36482 | Family 12 Diversity Ratio | 82492-0 |
| 36483 | Family 13 Diversity Ratio | 82491-2 |
| 36484 | Family 14 Diversity Ratio | 82490-4 |
| 36485 | Family 15 Diversity Ratio | 82489-6 |
| 36486 | Family 16 Diversity Ratio | 82488-8 |
| 36487 | Family 18 Diversity Ratio | 82487-0 |
| 36488 | Family 19 Diversity Ratio | 82486-2 |
| 36489 | Family 20-1 Diversity Ratio | 82485-4 |
| 36490 | Family 24-1 Diversity Ratio | 82484-7 |
| 36491 | Family 25 Diversity Ratio | 82483-9 |
| 36492 | Family 27 Diversity Ratio | 82482-1 |
| 36493 | Family 28 Diversity Ratio | 82481-3 |
| 36494 | Family 29 Diversity Ratio | 82480-5 |
| 36518 | Family 30 Diversity Ratio | 82479-7 |
| 36495 | Interpretation | 69047-9 |
| 36496 | Reviewed by | 18771-6 |
Forms
TCR V beta Spectratyping Assay Patient Information (T719) in Special Instructions