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Test Code NKSP Natural Killer (NK)/Natural Killer T (NKT) Cell Subset Panel, Blood

Useful For

Quantitation of natural killer (NK)/natural killer T (NKT) cell subsets as well as quantitation of specific cell-surface and intracellular proteins required for NK cell function

Reporting Name

NK/NKT Subset Panel

Specimen Type

WB Sodium Heparin

Advisory Information

Mayo Lab Director/Consultant approval is required prior to ordering test, call 800-533-1710.


A minimum number of CD16+CD56+ NK cell events (as measured by flow cytometry in the laboratory) are required to perform the NKSP test. Please be advised that any sample that does not meet this minimum requirement will have the NKSP test converted to QNKS / Natural Killer (NK)/Natural Killer T (NKT) Cell Subsets, Quantitative. Further, a minimum CD45 lymph count (as measured by flow cytometry in the laboratory) is required to report the NKSP test. If that requirement is not met (eg, patients with severe lymphopenia), the test will be canceled and an alternate test will be suggested (TBBS / Quantitative Lymphocyte Subsets: T, B, and Natural Killer (NK).

Shipping Instructions

Send specimen Monday through Thursday only. Specimen must arrive within 24 hours of draw and by 10 a.m. on Friday. Draw and package specimen as close to shipping time as possible. Ship specimen overnight.

Necessary Information

Ordering physician's name and phone number are required.

Specimen Required

For serial monitoring, we recommend that specimen draws be performed at the same time of day.


Container/Tube: Sodium heparin

Specimen Volume: 5 mL

Collection Instructions: Send specimen in original tube. Do not aliquot.

Specimen Minimum Volume

1.1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
WB Sodium Heparin Ambient 30 hours GREEN TOP/HEP

Reject Due To

Gross hemolysis Reject
Gross lipemia OK

Reference Values

The appropriate age-related reference values will be provided on the report. Pediatric reference values are not available and therefore, interpretation will be based on adult ranges with appropriate cautionary statements in the interpretation.

Day(s) and Time(s) Performed

Monday through Friday

Do not send specimen after Thursday. Specimen must be received by 10 a.m. on Friday.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information



86356 x3

LOINC Code Information

Test ID Test Order Name Order LOINC Value
NKSP NK/NKT Subset Panel In Process


Result ID Test Result Name Result LOINC Value
NKSPI NK/NKT Interpretation 69426-5
NKA45 Total Lymphs (CD45+) 27071-0
NKP3N % Total CD45+CD3- cells 89311-5
NKA3N Total CD45+CD3- cells 89313-1
NKP % Total NK Cells 8112-5
NKA Total NK Cells 9728-7
NKPCY % Cytotoxic NK (CD16++CD56+) 42189-1
NKACY Cytotoxic NK (CD16++CD56+) 42188-3
NK56P % Cytokine-Producing NK (CD56++) 8133-1
NK56A Cytokine-Producing NK (CD56++) 14113-5
NKPL3 % Total CD45+CD3+ Cells 56907-9
NKAL3 Total CD45+CD3+ Cells 89312-3
NKTP % NKT cells (CD3+CD56+) 17135-5
NKTA NKT cells (CD3+CD56+) 26858-1

Method Name

Flow Cytometry

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.


If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.