Sign in →

Test Code IFEU Immunofixation Electrophoresis, Urine

Important Note


Test Request Sample Type Test Code Description
Quantitiative Bence Jones 24hr urine UPEP Urine Electrophoresis includes Quantiative Bence Jones if present.
Qualitative Bence Jones 24 hr urine UPEP Immunofixation Electrophoresis (IFEU) is done if indicated by pathologist review.
Bence Jones (not specified) 24 hr urine UPEP See above entries.
Bence Jones (not specified) random UPEP

Note: Per September 2010 LabWire, random urine testing is not the optimal sample for monoclonal studies. A 24 hour sample is recommended. 


Performing Laboratory

Bronson Laboratory Services-Chemistry

Reference Values

An interpretive report will be provided.


Immunofixation Includes identification of monoclonal gammopathies and pathologist interpretation.


Immunofixation electrophoresis of urine is most often ordered to evaluate a monoclonal globulin detected in a protein electrophoresis or to delineate a possible lymphoproliferative process, particularly myeloma. This procedure will characterize the specific light- and heavy-chain components of a monoclonal protein. Pathologist may order quantitative immunoglobulins to further evaluate any monoclonal protein detected.

Specimen Requirements

Specimen Type: Urine, 24 hour. Entire collection.

Container/Tube: Clean, plastic urine container

Specimen Volume: 30 mL aliquot from well mixed 24-hour urine.

Collection Instructions:

1. No preservative. Refrigerate specimen during collection.

2. Random urine collection is not acceptable.

Additional Information:

1. 24-Hour volume is required.

2. Starting and ending dates/times of collection are required.

3. Follow instructions in Patient Instructions for 24-Hour Collection under Special Instructions.

Processing Instructions:

1. Measure and record total volume.

2. Mix entire collection well and pour off a 30.0 mL aliquot.

Specimen Transport Temperature


Day(s) Test Set Up

Tuesday, Thursday

Test Classification and CPT Coding

86335-Immunofixation electrophoresis

84156-Protein, total

Please Note: Additional CPT and professional fee for pathologist’s interpretation may be applied.

Special Instructions

BMH Laboratory Only

  1. the proper collection duration and volume entered
  2. a urine cup placed in the 24 hour bin in the Accession refrigerator
  3. an aliquot poured off into a false bottom tube for a urine total protein for chemistry
  4. an aliquot that is a minimum of 12 mL for wet chemistry placed in the KWET rack ( 12 mL urine tubes) or blue container (cups)