Test Code B12 Vitamin B12, Serum or Plasma
Additional Codes
Epic Order Code: | LAB67 |
Sunquest Order Code: | B12 |
Specimen Required
Container Type: |
Optimal: Gold Top Acceptable: Mint (preferred for STAT requests), Red Top or Green Lithium Heparin.
|
Optimal Collection Volume: |
4.5 mL; Full Tube |
Collection Instructions: |
Gently invert sample 5-6 times after collection. |
Processing Instructions: |
1. Allow serum sample to clot for 30 minutes. 2. Centrifuge specimen within 2 hours of collection. 3. Specimen tubes without a gel barrier should have the serum or plasma aliquoted to a false bottom container after centrifugation. 4. Keep serum/plasma refrigerated until testing can be performed. |
Useful For
This assay is used as the investigation of nutritional and macrocytic anemias caused by a deficiency of vitamin B12.
Method Name
Electrochemiluminescence Immunoassay
Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Serum/Plasma | Refrigerated | 7 days |
Ambient | 2 hours | |
Frozen | 2 months |
Rejection Due To
Hemolysis | Mild OK; Moderate Reject; Gross Reject |
Lipemia | Mild OK; Moderate OK; Gross Reject |
Icterus | Mild OK; Moderate OK; Gross Reject |
Other | N/A |
Post Processing Specimen Type
Preferred: Serum
Alternate: Plasma
Specimen Minimum Volume
Collecting minimum volumes can result in a need for sample recollection, and/or a delay in results. Minimum volumes are subjective and cannot account for all aspects of specimen and testing needs. Refer to the Specimen Required section for optimal volumes for laboratory specimens. Contact the Bronson Laboratory if complex collection exceptions occur that require more information.
Minimum Volume: | 1 mL whole blood, 0.5 mL serum or plasma |
Performing Laboratory
Bronson Laboratory, Chemistry - Paw Paw
Day(s) and Time(s) Performed
24/7
Reference Values
Test ID | Reference Range | Units |
---|---|---|
B12 | 211-946 | pg/mL |
LOINC Code Information
2132-9
CPT Code Information
82607
Sample Retention Time
7 days