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Test Code AJO Jo-1 Antibody, Serum

Additional Codes

Epic Code


Performing Laboratory

Bronson Methodist Hospital Laboratory

Reference Values

<1.0 AI (Antibody Index) = Negative

>1.0 AI (Antibody Index) = Positive


Antibodies to Jo-1 are present in 20-55% of cases with poly-dermatomyositis.

Specimen Requirements

Specimen Type: Serum

Container/Tube: Gold top serum gel-Plain,
red-top tube is also acceptable.
Specimen Volume: 1 mL

Specimen Minimum Volume: 0.5 mL

Specimen Collection and Handling: 
Separated serum/plasma should remain at 22 ͦC for no
longer than eight hours.  If assays will not be completed
within eight hours, serum/plasma should be refrigerated
(2-8 ͦC).  For storage beyone 72 hours, freeze
sample at -20 ͦC.  Avoid repeated freezing and

Specimen Transport Temperature


Day(s) Test Set Up

Monday through Friday

Test Classification and CPT Coding



Multiplex flow immunoassay

Please see the January 2019 LabWire link under the "Resources" section of this page for infomation on methodology change and the Connectiive Tissue Disease cascade.