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HelpTest Code ADM13 ADAMTS13 Activity and Inhibitor Profile, Plasma
Reporting Name
ADAMTS13 Activity and Inhibitor ProfileUseful For
Assisting with the diagnosis of congenital or acquired thrombotic thrombocytopenic purpura
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ADMIS | ADAMTS13 Inhibitor Screen | No | No |
| ADMBU | ADAMTS13 Inhibitor Bethesda Titer | No | No |
Testing Algorithm
Testing begins with ADAMTS13 activity assay to evaluate the percent activity. If the ADAMTS13 activity assay is less than 30%, an inhibitor screen will be performed to look for specific ADAMTS13 inhibition. If specific inhibition is apparent, the titer of the inhibitor will be determined.
Method Name
ADMFX: Fluorescence Resonance Energy Transfer (FRET)
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Plasma Na CitShipping Instructions
Send both vials in the same shipping container.
Specimen Required
See Coagulation Guidelines for Specimen Handling and Processing in Special Instructions.
Patient Preparation: Fasting preferred
Collection Container/Tube: Light-blue top (citrate)
Submission Container/Tube: Plastic vials
Specimen Volume: 2 mL in 2 plastic vials each containing 1 mL
Collection Instructions:
1. Specimen must be drawn prior to replacement therapy.
2. Spin down, remove plasma, and spin plasma again.
3. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or, ideally, ≤-40° C.
Additional Information:
1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.
2. If priority specimen, mark request form, give reason, and request a call-back.
3. Each coagulation assay requested should have its own vial.
Specimen Minimum Volume
2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma Na Cit | Frozen | 14 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Special Instructions
Reference Values
ADAMTS13 ACTIVITY ASSAY
≥70%
ADAMTS13 INHIBITOR SCREEN
Negative
ADAMTS13 BETHESDA TITER
<0.4 BU
Day(s) and Time(s) Performed
Monday through Sunday; Varies
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
85397-ADAMTS13 activity assay
85335-ADAMTS13 inhibitor screen assay (if appropriate)
85335-ADAMTS13 Bethesda titer (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ADM13 | ADAMTS13 Activity and Inhibitor Profile | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 61211 | ADAMTS13 Activity Assay | 53622-7 |
| 34586 | ADAMTS13 Interpretation | 69049-5 |
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ADMFX | ADAMTS13 Activity Assay | No | Yes |
| ADMIN | ADAMTS13 Interpretation | No | Yes |
Forms
1. Coagulation Patient Information (T675) in Special Instructions
2. If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Coagulation Test Request (T753)
-Renal Diagnostics Test Request (T830)